几种试剂对肌酐测定结果影响探析.docVIP

几种试剂对肌酐测定结果影响探析[摘要] 目的 探讨全自动生化分析仪项目检测过程中，葡萄糖和肌酸激酶及其同工酶试剂对肌酐测定结果的影响。方法 用“中生北控生物科技股份有限公司”生产的质控血清当做标本，先单独测定肌酐，然后再分别先测定葡萄糖和肌酸激酶及其同工酶后测定肌酐。结果 先测定葡萄糖和肌酸激酶及其同工酶后测定肌酐所得结果与单独测得的肌酐结果比较有显著差异(p0.01)。结论 全自动生化分析仪项目检测过程中，用先测定葡萄糖和肌酸激酶及其同工酶后测定肌酐的检测顺序时，葡萄糖和肌酸激酶及其同工酶试剂对肌酐的测定结果有影响，可使肌酐测定结果偏高。 [关键词] 葡萄糖和肌酸激酶及其同工酶； 肌酐； 化学污染 [中图分类号] R445 [文献标识码] B [文章编号] 1005-0515（2012）-01-024-01 [Abstract] Objective To investigate the automatic biochemical analyzer project testing process, glucose and creatine kinase isoenzyme reagent for creatinine determination results. Methods “BIOSINO Biotechnology Co.,Ltd.”as the production of quality control serum samples, the first determination of creatinine alone, and then glucose were measured before and after the determination of creatine kinase and its isoenzymes creatinine. Results The first determination of glucose and creatine kinase and its isoenzymes were measured after single creatinine results measured with the creatinine result there was a significant difference(p0.01).Conclusion The automatic biochemical analyzer during the test project, with the first determination of glucose and creatine kinase and its isoenzymes were measured after the test sequence creatinine, glucose, and creatine kinase isoenzyme reagent for the determination of creatinine results effects can result high creatinine determination. [Key words] Glucose and creatine kinase and its isoenzymes; Creatinine; Chemical pollution 随着全自动生化分析仪的普及，给临床检验工作带来了方便，提高了工作效率。但由于仪器常规清洗程序无法有效清除污垢以及共同使用试剂针、样本针、搅拌棒、比色杯等造成交叉污染；项目检测顺序排列不当等等都会增加试剂间化学污染的可能性，引起检测结果偏低或偏高。我们在日常工作中就发现先测定葡萄糖和肌酸激酶及其同工酶后测定肌酐，可造成肌酐结果偏高，可能为试剂间的化学污染。 1 材料与方法 1.1 仪器与试剂 日立全自动生化分析仪。葡萄糖(氧化酶法)和肌酸激酶及其同工酶(NAC法)和肌酐(氧化酶法)试剂均为四川迈克公司生产。 1.2 标本 用“中生北控生物科技股份有限公司”生产的质控血清当做标本：用稀释器准确吸取3ml蒸馏水，加入瓶中，盖上瓶塞，静止10分钟，轻轻颠倒10次，使其完全溶解混匀。 1.3 方法 1.3.1 单独测定肌酐 用日立全自动生化分析仪将质控血清重复测定20次，结果为(157±6)umol/L。 1.3.2 分别先测定葡萄糖和肌酸激酶及其同工酶,后测定肌酐 用日立全自动生化分析仪将质控血清重复测定20次，测定结果分别为(389±5)umol/L、(405±7)umol/L和(393±6)umol/L。 1.3.3 统计学方法 用SPSS11.0软件处