异氟醚预处理延迟相对兔缺血再灌注心肌Bcl-2蛋白表达及IL-10水平影响.doc

异氟醚预处理延迟相对兔缺血再灌注心肌Bcl-2蛋白表达及IL-10水平影响【摘要】目的：探讨异氟醚预处理延迟相对缺血再灌注心肌的保护作用及其机制。方法：30只健康新西兰雄性大白兔随机分成3组：假手术组(C组)、缺血再灌注组(I/R组)、2.0％异氟醚预处理延迟相组(S组)，每组10只。C组仅开胸160 min，I/R组行左冠脉阻断40 min，再灌注120 min，S组吸入2.0％异氟醚2小时，24小时后处理同I/R组。各组分别于左冠脉阻断前20 min（T1）、左冠脉阻断20 min（T2）、左冠脉阻断40 min（T3）、再灌注1小时h（T4）、再灌注2小时（T5）5个时点抽血测血清IL-10水平。再灌注结束后免疫印迹法测心肌Bcl-2表达水平，用伊文思蓝和TTC染色测心肌梗死面积。结果：与I/R组比，S组IL-10水平增高(P0.05)，Bcl-2表达增高(P0.05)，心肌梗死面积减少(P0.05)。结论：异氟醚预处理延迟相通过上调心肌Bcl-2表达和IL-10生成来减轻缺血再灌注损伤发挥保护作用。 【关键词】异氟醚；预处理延迟相；心肌缺血再灌注；Bcl-2；IL-10 文章编号：1009-5519（2007）16-2371-02 中图分类号：R614 文献标识码：A Effects of isoflurane delayed preconditioning on Bcl-2 protein expression and IL-10 level in ischemiareperfusion myocardium of rabbit RAN Ke，DUAN Kai-ming，ZOU Ding-quan，et al. (1.Department of Anesthesiology,The Second Xiangya Hospital,Zhongnan University,Changsha 410011,China;2.Department of Anesthesiology,The Third Xiangya Hospital,Zhongnan University,Changsha 410013 China) 【Abstract】Objective: To investigate the protective effect of isoflurane delayed preconditioning on myocardial ischemia reperfusion injury and the potential mechanisms in rabbit. Methods:Thirty New Zealand male white rabbits were randomly assigned to 3 groups：(1)Control group;(2)I/R group;(3)2.0％ isoflurane group. Group 3 was exposed to 2.0% isoflurane-100% oxygen for 2 h. Group 1 and group 2 were exposed 2 h to 100% oxygen serving as untreated controls. Twenty-four hours later group 2 and group 3 underwent 40 min of coronary occlusion followed by 2 h of reperfusion. Blood samples were taken from arterial line at 20min before occlusion(T1)、20min after occlusion(T2)、40min after occlusion(T3)、1h after reperfusion(T4) and 2h after reperfusion(T5) for determination of the plasma level of IL-10. At the end of the reperfusion， infarct size(IS) and area at risk(AAR) were defined by Evans and TTC staining. The heart was harvested and level of the Bcl-2 expression was determined by Western Blot analysis. Results: The Bcl-2 level of group 3 was signif