施万细胞对共培养神经干细胞增殖及分化影响.doc

施万细胞对共培养神经干细胞增殖及分化影响[摘要]目的：探讨施万细胞诱导神经干细胞增殖与分化的影响因素。方法：大鼠胚胎神经干细胞单独培养及与新生SD大鼠施万细胞共培养，观察神经干细胞的形态变化，并分别检测特异性标志蛋白S-100、NF和GFAP的表达。结果：相差显微镜下可见共培养组大部分神经干细胞伸出多个细长突起；与单独培养组相比，免疫荧光染色显示共培养组NF表达阳性细胞数多；GFAP表达阳性细胞数少。结论：施万细胞可促进共培养的神经干细胞增殖，并可诱导神经干细胞向神经元方向分化。 [关键词]施万细胞；神经干细胞；增殖；分化 [中图分类号]R329.2 [文献标识码]B[文章编号]1673-7210（2007）09（a）-098-02 Effects of Schwann Cells on the generation and differentiation of neural stem cells in vitro LIU Zhong,ZENG Shui-lin,LI Feng-fei,JIANG Yu,ZHU Jian-bao (Institute of Neurobiology, Southeast University,Nanjing 210009,China) [Abstract] Objective：To explore the influencing factors of Schwann Cells in promoting neural stem cells to generate and differentiate in vitro.Methods：Neural stem cells were dissociated and cloned from the cerebral subventricular zone of embryo rats，Schwann Cells were also dissociated and purified simultaneously from the sciatic nerves of newborn rats．Then neural stem cells were cultured separately and Co-cultured with Schwann Cells．The expression of protein S-100, neurofilament protein (NF) and glial fibrillary acidic protein (GFAP) of the neural stem cells were detected by immunofluorescence．The morphological changes of neural stem cells were examined with contrast phase microscope．Results：The co-cultured groups had more positive Cells of protein S-100 and NF,and less positive cells of GFAP than the group cultured separately. The nervous process of neuron-like cells in Co-cultured groups were obviously longer and more than that in separately cultured group．Conclusion：Schwann Cells can promote the generation and differentiation of neural stem cells in vitro． [Key words]Schwann Cells;Ｎeural stem cells;Generation;Differentiation 神经干细胞(neural stem cells,NSCs)是指存在于神经系统内的具有自我复制能力和多向分化潜能的细胞[1]。研究证明，NSCs不仅存在于哺乳动物胚胎脑组织，而且存在于发育和成熟的CNS，如大脑皮质、海马、脑室下区等可分离出NSCs，这不仅改变了成年动物的神经细胞不能分裂的传统观点，而且在研究脑的发育和分化以及相关疾病治疗上均具有重大价值[2,3]。 施万细胞(Schwann Cells，SCs)作为周围神经系统的主要胶质细胞，包绕周围神经轴突形成有髓或无髓神经纤维，能分泌多种细胞因子与营养因子、细胞外基质及细胞黏附因子，有利于维持神经元的生存和N