早期诊断宫颈上皮内病变及宫颈鳞癌标记物――Ki-67.doc

早期诊断宫颈上皮内病变及宫颈鳞癌标记物――Ki-67[摘要] 目的：探讨宫颈上皮内病变及宫颈鳞癌的标记物――Ki-67的表达及意义。方法：应用免疫组化S-P法检测14例宫颈鳞癌、77例宫颈上皮内病变、23例正常宫颈组织中Ki-67的表达水平，并加以分析。结果：①Ki-67阳性表达率在宫颈鳞癌中最高(100％)，宫颈上皮内病变次之(93.33％～100％)，正常宫颈组最低(39.13％)。宫颈上皮内病变及宫颈鳞癌与正常组比较，差异有统计学意义(P＜0.01)。②从正常宫颈组织、宫颈上皮内病变到宫颈鳞癌，Ki-67的表达强度逐渐增强，两者呈正相关(r=0.770 8)。结论：Ki-67参与宫颈上皮内病变、宫颈鳞癌的发生。作为早期诊断宫颈上皮内病变及宫颈鳞癌的标记物, Ki-67的检测可提高宫颈鳞癌的早期诊断率。 [关键词] Ki-67；早期诊断；宫颈上皮内病变；宫颈鳞癌 [中图分类号] R737.33[文献标识码]A [文章编号]1673-7210（2008）08（c）-034-03 Ki-67――the marker of early diagnosis of cervical intraepithelial neoplasia and cervical squamous carcinoma ZHANG Ya-jing, CHEN Rong-Hua (Department of Pathology, Affiliated Chinese Medicine Hospital of Xinjiang Medical University, Urumqi830000, China) [Abstract] Objective: To investigate the expression and significance of Ki-67――a marker of the cervical intraepithelial neoplasia and cervical squamous carcinoma. Methods: Immunohistochemical S-P assay was used to detect the expression of Ki-67 in 14 cases cervical squamous carcinoma and 77 cases cervical intraepithelial neoplasia and 23 cases normal cervix. Results: ①The positive expression rates of Ki-67 in cervical squamous carcinoma, cervical intraepithelial neoplasia and normal cervix were 100%, 93.33%～100% and 39.13% respectively, and it showed a significant difference compared with normal cervix group(P＜0.01). ②The expression intensity order of Ki-67 was normal cervix, cervical intraepithelial neoplasia and cervical squamous carcinoma from down to up，and they were in a positive correlation(r=0.770 8). Conclusion: Ki-67 involves in the occurrence of cervical intraepithelial neoplasia and cervical squamous carcinoma. As a marker of early diagnosis of cervical intraepithelial neoplasia and cervical squamous carcinoma, the determination of Ki-67 could enhance the early diagnosis rate. [Key words] Ki-67; Early diagnosis; Cervical intraepithelial neoplasia; Cervical squamous carcinoma 宫颈癌是常见的妇科恶性肿瘤之一，在全球妇女恶性肿瘤中排第二位，极大地威胁着妇女健康，早期诊断和早期治疗能降低其死亡率。子宫颈上皮细胞的不典