il鄄23在变应性鼻炎小鼠模型中的作用及机制的研究.pdfVIP

刘摇 阳等摇 IL鄄23在变应性鼻炎小鼠模型中的作用及机制的研究摇 第11期 ·1649 · doi:10.3969/ j.issn.1000鄄484X.2016.11.018 IL鄄23在变应性鼻炎小鼠模型中的作用及机制的研究淤 于 刘摇 阳摇 余海静 摇 刘摇 争摇 (同济医院耳鼻咽喉头颈外科,武汉 430030) 摇 摇 中图分类号摇 R765 郾21摇 摇 文献标志码摇 A摇 摇 文章编号摇 1000鄄484X(2016)11鄄1649鄄04 [摘摇 要]摇 目的:探讨IL鄄23在变应性鼻炎小鼠模型中的作用及机制的研究。 方法:应用卵清蛋白(OVA)致敏建立小鼠 变应性鼻炎模型,给予抗IL鄄23p19单克隆抗体干预,计量抗原激发后小鼠搔鼻数量。 应用 HE染色方法检测IL鄄23对小鼠鼻 黏膜炎症影响,应用ELISA法检测小鼠灌洗液中IL鄄4、IFN鄄酌及IL鄄17A含量。 应用PCR方法检测鼻黏膜中FOXP3 的含量。 结 果:治疗后抗IL鄄23p19抗体组小鼠搔鼻症状相对于抗体对照组明显减轻(P0郾05),与PBS组比较,OVA组小鼠鼻黏膜炎症细 胞浸润明显(P0郾01),鼻腔灌洗液中嗜酸性粒细胞数明显升高(P0郾05);抗IL鄄23p19抗体治疗后,小鼠鼻黏膜炎症细胞浸润 显著降低(P0郾05),鼻腔灌洗液中嗜酸性粒细胞数明显降低(P0郾05);抗IL鄄23p19抗体治疗后,IL鄄4 及 IL鄄17A含量较IgG 抗体对照组降低(P0郾05),IFN鄄酌含量不变(P0郾05)。 抗 IL鄄23p19 抗体治疗后,FOXP3 含量较 IgG抗体对照组增高(P 0郾05)。 结论:抗IL鄄23p19抗体可有效治疗小鼠变应性鼻炎,其机制可能是抑制Th17细胞的增殖并且促进调节性T细胞的功能。 [关键词]摇 IL鄄23;变应性鼻炎;IL鄄17A Effect of IL鄄23 in a model of allergic rhinitis and its mechanism LIU Yang,YU Hai鄄Jing,LIU Zheng郾Department of Otolaryngology鄄Head Neck Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan430030,China [Abstract]摇 Objective:To explore the effect of IL鄄23 inthetreatment of allergicrhinitisinmice郾Methods:Amodel of allergic rhinitiswas establishedwith Ovalbumin(OVA),and then the mice were treated with anti鄄IL鄄23p19郾The nasal rubs were counted郾HE staining was performed and thenumberoftotalinflammatory cellsandeosinophilswascountedinthenasallavage郾Thecontentof IL鄄4, IFN鄄酌 and IL鄄17A were measured by ELISA郾The amount of FOXP3 in the local mucosa was measured by Real鄄time PCR method郾Results:Compared to IgG control group,after the treatment of anti鄄IL鄄23p19,the nasal rubs were decreased (P0郾05). Compared toPBSgroup,thetotalinflammatory cellsinthenasalmucosaandNLFeosinophilsshowedsignificantdifferencefortheOVA group(P0郾01andP0郾05 respectively),but after the anti鄄IL鄄23p19 treatment,they were significantly decreased (P0郾05).IFN鄄酌 was not differe