大叶相思下胚轴离体培养再生植株的研究.PDFVIP

林 业 科 学 研 究　2008, 2 1 ( 3) : 403～406 F ores t R esea rch 　　文章编号 : 100 11498 (2008) 大叶相思下胚轴离体培养再生植株的研究 1, 2 1 1 1 刘娟旭 , 刘 玲 , 王 静 , 余义勋 ( 1. 华南农业大学园艺学院 ,广东 广州 　510642; 2. In stitu te of Food and A gricu ltu ral Sciences, Un iversity of Florida, Apop ka, FL　327038504 , U. S. A. ) 关键词 :大叶相思 ; 下胚轴 ; 植物生长调节剂 ; 植株再生 中图分类号 : S792. 99　　　　　文献标识码 : A Plan t Regenera tion from Hypocoty ls of A ca c ia au r icu liform is Cultured in vitro 1, 2 1 1 1 L I U J uan x u , L I U L ing , WAN G J ing , YU Yix un ( 1. College of Horticu ltu re, Sou th Ch ina A gricu ltu ral Un iversity, Guangzhou 510642 , Guangdong, Ch ina; 2. In stitu te of Food and A gricu ltu ral Sciences, Un iversity of Florida, Apopka, FL　327038504 , U. S. A ) A b stract: The regenerated p lan tlets ofA cacia au ricu lif orm is were ob tained by the m ethod of redifferen tiation of the callu s from the hypocotyls exp lan ts. The effects of p lan t regu lator compo sition s on the induction of callu s and the differen tiation of adven titiou s bud s in vitro cu ltu re were studied. The op tim um m edium for callu s induction wa s M S - 1 - 1 m edium con tain ing 1. 0 or 1. 5 m g ·L 2 , 4 D and 0. 5 m g ·L KT and 100% the callu s induction frequency wa s