自记录动物握杠垂吊耐力测试仪-中国试验动物学报.DOCVIP

自记录动物挂杠持久度测试装置的研制 惠恩健，葛尔宁，盛振华，黄真 (浙江中医药大学 杭州 310053) [摘要] 目的方法结Development of an experimental instrument for testing the Endurance of an animal hanging on a bar HUI En–jian，GE Er–ning，SHENG Zhen-hua，HUANG Zhen (Zhejiang Chinese Medical University, Hangzhou 310053,China) [Abstract] Objective To develop an instrument that detects the endurance of an animal hanging on a bar during medical and pharmaceutical scientific experiments. Methods One pair of opto sensors were used for signal conversion. A solenoid valve device was used for water level. A 51-Series microcontroller was used to control the experimental setup and record the results. Results We have developed a microcontroller-based experimental instrument that can automatically detects the endurance of an animal hanging on a bar during medical and pharmaceutical scientific experiments. Our experimental results with 28 mice indicated that the applicability of the instrument is good. Conclusion The instrument provides an innovative tool for detecting the endurance of an animal . [Key words] Hanging on a bar; Medical and pharmaceutical research; Opto sensors; Microcontroller. 动物用爪挂杠持久度实验同属于静态抗疲劳实验[1]，可用在抗疲劳、抗抑郁等药物的研制及相关医学研究中。当动物在一个封闭的水池中游泳时，因恐惧或体力不支往往会寻找可以抓住的支撑点，而当四爪及臂不能支撑整个身体重量时又会放弃挂杠掉入水中，如此重复多次，最终放弃抓杠。挂杠时间和次数可用于评价研制药物的药效及相关医学作用。 这种让动物自然抓杠挂臂的实验方式可用于动物静态疲劳的检测，采用该方法测试动物抗疲劳能力目前国内外均未见报道，类似动物抗疲劳实验目前在业界有采用站线式、站杠式[1]等方法， 对动物来讲，站立姿势较易做到，持久度可较长，一次实验时间相对较长，有时需要24 h以上，本文所述方法与前两种的不同在于：本研究以动物多臂挂杠的姿势进行疲劳实验，相比前者难度增加，持久度降低，相应实验时间缩短。实验常用动物如小白鼠等的臂力有限，一次挂杠时间不会很长，如此可在水