雌雄文昌鱼同工酶的表型差异currentzoology.pdf

动物学报 　50 ( 1) :62 - 67 , 2004 A cta Zoologica S inica 　　　　　 　 雌雄文昌鱼同工酶的表型差异 庞秋香　张士璀 　王长法　王昌留　张永忠 中国海洋大学海洋生物系, 青岛 266003 摘　要 　本文应用聚丙烯酰胺凝胶电泳结合生化染色方法分析了雌雄文昌鱼中苹果酸酶、苹果酸脱氢酶、酸性 磷酸酶和酯酶四种同工酶的酶谱。首次发现苹果酸酶、苹果酸脱氢酶和酸性磷酸酶表型在文昌鱼雌性和雄性个 体之间存在差异 , 而在同一性别不同个体之间无差异。酯酶表型较复杂 , 不但在不同性别个体之间而且在同一 性别不同个体之间都出现一定差异 [动物学报 50 ( 1) : 62 - 67 , 2004 ] 。 关键词 　文昌鱼 　苹果酸酶 　苹果酸脱氢酶 　酸性磷酸酶 　酯酶 Sexrelated differences in isozymic phenotypes in amphioxus Branchiostoma belcheri tsingtauense PAN G QiuXiang , ZHAN G ShiCui , WAN G ChangFa , WAN G ChangLiu , ZHAN G YongZhong Department of Marine Biology , Ocean University of China , Qingdao 　266003 , Shandong , China Abstract 　Four isozymes , i. e. malic enzyme , malate dehydrogenase , acid phosphatase and esterase , in amphioxus B ran chiostom a belcheri tsingtauense , were analyzed using polyacrylamide gel electrophoresis ( PA GE) and biochemical staining. It was found that ( 1) the electrophoretic patterns of the isozymes showed sexrelated differences between male and female amphioxus ; (2) the electrophoretic patterns of malic enzyme , malate dehydrogenase and acid phosphatase did not show interindividual difference in the same sex ; (3) however , the electrophoretic patterns of esterase showed some differences in both sexes as well as in different individuals of the same sex [Acta Zoologica Sinica 50 ( 1) : 62 - 67 , 2004 ]. Key words 　Amphioxus , B ranchiostom a belcheri tsingtauense , Malic enzyme , Malate dehydrogenase , Acid phosphatase , Esterase 　　同工酶的形成是生物在进化过程中对代谢适应 研究报道 ( Tung et al. , 1958 ; Fang et al. , 2003 ; 的结果。同一种群中的不同个体、不同性别、同一 童第周等 , 1964 ;