弱光胁迫下钙对番茄叶片钙组分和活性氧代谢的影响毕业论文.doc

弱光胁迫下钙对番茄叶片钙组分和活性氧代谢的影响毕业论文 目 录 中文摘要（关键词）┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉1 英文摘要（关键词）┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉2 前言┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉3 1材料与方法 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉6 .1实验材料和实验设计 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉6 1.2测定项目及方法 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉6 1.2.1Ca2+测定方法 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉6 1.2.2超氧自由基(O2-)产生速率测定 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉6 1.2.3丙二醛(MDA)含量测定方法 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉7 1.2.4超氧化物歧化酶(SOD)活性测定方法 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉7 1.2.5过氧化物酶(POD)活性测定方法 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉7 2.3 数据处理和分析┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉7 结果和分析┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉7 .1钙对弱光下番茄叶片钙形态的影响 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉7 .2钙对弱光胁迫下番茄叶片活性氧代谢的影响┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉ 2.2.1Ca2+对弱光胁迫下番茄叶片超氧阴离子产生速率的影响 ┉┉┉┉┉┉┉┉┉┉┉8 2.2.2 Ca2+对弱光胁迫下番茄叶片MDA含量的影响┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉9 2.2.3 Ca2+对弱光胁迫下番茄叶片SOD活性的影响┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉10 2.2.4 Ca2+对弱光胁迫下番茄叶片POD活性的影响┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉10 3讨论┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉1 3.1 钙对弱光下番茄叶片钙形态的影响┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉11 3.2弱光胁迫对活性氧代谢的影响┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉12 3.3钙对弱光下番茄叶片活性氧代谢的影响┉┉┉┉┉┉┉┉┉┉┉┉┉┉12 4结论┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉1 参考文献 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉1 致谢 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉1 为了研究钙对番茄耐弱光特性的调控作用，以番茄(Lycopersicon esculentum Mill)品系W为试材研究了弱光(25%自然光强)胁迫条件下叶片中H2O-Ca的变化是总钙浓度改变的主要原因说明水溶钙可能在调节光合方面发挥着重要的作用降低了番茄叶片超氧阴离子的产生速率和MDA含量，提高了SOD活性和POD活性Ca2+通过提高保护酶活性、降低膜质过氧化水平来提高番茄植株，进一步防止植株伤害。 Abstract In order to investigate the regulating mechanism of calcium on tomato low light tolerance, leaves of one tomato low light tolerance strain W (Lycopersicon esculentum Mill) were used to study the changes of Calcium components and Metabolism of reactive oxygen species (ROS) in leaves after the tomato were treated by calcium under the 25% nature light intensity .The results were as follows: Calcium decreased under low light stress of total calcium content in tomato leaves, leaf in H2O - Ca change is a major cause of total calcium concentration change, suggests that water soluble calcium may play an important role in regulating photosynthesis. Under weak light stress calcium reduces the