反油酸对人脐静脉内皮细胞活力的影响.pdf

372 2010, Vol. 31, No. 19 食品科学 ※营养卫生 于化泓，李湘梅，刘 蓉，邓泽元* ，李 静，范亚苇，胡蒋宁 (南昌大学 食品科学与技术国家重点实验室，江西 南昌 330047) ：目的：观察反油酸对人脐静脉内皮细胞(HUVEC)增殖活力的影响。方法：采用 50 、100、200 、400 、 600 μmol/L 等 5 个浓度的反油酸作用于人脐静脉内皮细胞，以正常组为阴性对照组，NaOH 组(200 μmol/L)为阳性对 照组，MTT 法和台盼蓝排斥实验检测其对内皮细胞的毒性。结果显示：反油酸组内皮细胞增殖活力明显低于正常 组，且高浓度组(400、600 μmol/L)对细胞增殖抑制率明显高于低浓度组(50 μmol/L)；而NaOH 组与正常组比较却无 明显差异。此外，反油酸组活细胞率降低。结论：反油酸对内皮细胞有明显毒副作用，其生长抑制率与反油酸 浓度和作用时间呈正相关。 ：反油酸；人脐静脉内皮细胞；M T T 法 Effect of Oleic Acid on Proliferation of Human Umbilical Vein Endothelial Cells YU Hua-hong ，LI Xiang-mei ，LIU Rong ，DENG Ze-yuan* ，LI Jing ，FAN Ya-wei ，HU Jiang-ning (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China) Abstract：Objective: To observe the effect of oleic acid on the proliferation of human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were treated with oleic acid at concentrations of 50, 100, 200, 400 μmol/L and 600 μmol/L (in combination with NaOH at a final concentration of 200 μmol/L), respectively. The normal group receiving no treatment was served as the negative control, and the groups treated with NaOH at 200 μmol/L alone were used as the positive control. MTT assay and trypan blue exclusion tests were used to evaluate the toxicity of oleic acid on cells. Results: Compared with the normal group, oleic acid significantly inhibited the proliferation of HUVECs. In addition, compared with the group treated with oleic acid at a low concentration (50 μmol/L), an obvious strong inhibition effect on cell proliferation was observed in groups treated with oleic acid at high concentrations (400 μmol/L and 600 μmol/L). However, no significant difference was observed between the positive control and the normal group. Moreover, the