梓醇对大鼠慢性脑缺血白质损伤的保护作用 - 第三军医大学学报.doc

梓醇对大鼠慢性脑缺血白质损伤的保护作用 蔡其燕，陈兴书，姚忠祥（第三军医大学基础医学部组织学与胚胎学教研室，重庆，400038） 摘要：目的 初步探讨梓醇对慢性脑缺血所致白质损伤的保护作用及机制。方法 通过结扎大鼠双侧颈总动脉建立慢性脑缺血模型，同时腹腔注射梓醇予以治疗，然后利用LFB染色法和免疫组织化学技术分别观察梓醇对脑白质髓鞘以及MBP、CC-1和GFAP表达的影响。结果 缺血后脑白质髓鞘的LFB染色变浅，MBP和CC-1的表达下降，GFAP的表达升高；给予梓醇治疗后能明显减轻脑缺血造成的上述白质病理改变，即能减轻髓鞘损伤、抑制少突胶质细胞减少和星形胶质细胞增多。结论 梓醇可通过抑制炎症反应来保护慢性脑缺血所致的白质损伤。 关键词：梓醇；大鼠；慢性脑缺血；脑白质损伤 中图法分类号：R743.05 文献标识码：A Neuroprotective effect of Catalpol on white matter injury after chronic cerebral hypoperfusion in the rat Cai Qiyan, Chen Xingshu, Yao Zhongxiang （Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China） Abstract: Objective To detect the neuroprotective effect of catalpol and its action mechanism on white matter injury after chronic cerebral hypoperfusion in rats. Methods Chronic cerebral hypoperfusion was prepared by permanent ligation of bilateral common carotid arteries and the ischemic rats were treated by intraperitoneal injection of catalpol at the commencement of hypoperfusion. LFB staining and immunohistochemical method were used to investigate the effects of catalpol on the myelin sheath and the expressions of MBP、CC-1 and GFAP in the white matter,( respectively. Results The LFB staining of myelin sheath was reduced, the expressions of MBP and CC-1 were decreased, and the expression of GFAP was increased in the white matter after hypoperfusion. Catalpol treatment significantly alleviated the pathological changes of white matter following chronic cerebral hypoperfusion. It markedly attenuated the damage of myelin sheath, suppressed the decrease of oligodendrocytes and the increase of astrocytes in number. Conclusion Catalpol protects against the white matter injury under chronic cerebral hypoperfusion by moderation the inflammatory reaction. Key words: Catalpol; rat; chronic cerebral hypoperfusion; white matter injury Corresponding author: Yao Zhongxiang, Tel:86-23 E-mail: yaozhx@ 脑缺血是神经系统的多发病之一，与多种脑血管疾病如脑动脉硬化、血管性痴呆、老年性痴呆等密切相关。传统观念认为，中枢神