毒死蜱对斑马鱼胚胎氧化应激效应研究 - 北京玛格泰克科技发展有限公司.pdf

中国环境科学 2016,36(3) ：927~934 China Environmental Science 毒死蜱对斑马鱼胚胎氧化应激效应研究 张家禹,刘丽丽,李国超,余凯敏, 吕 鹏,闫艳春* ( 中国农业科学院研究生院,北京 100081) 摘要： 以斑马鱼为模型,研究了毒死蜱对斑马鱼胚胎形态学的影响,及其对胚胎的氧化应激和氧化损伤作用.将斑马鱼胚胎暴露在梯度浓度 的毒死蜱溶液中 96h 后,发现毒死蜱会造成斑马鱼胚胎严重畸形甚至死亡,其 96h 半致死浓度为 1.18mg/L.对氧化应激相关基因的表达及抗 氧化酶活性和丙二醛(MDA)含量进行检测,结果表明,在毒死蜱胁迫下抗氧化酶(SOD、CAT)活性降低,并且其编码基因(Cu/Zn-sod 、Mn-sod 、 cat) 的表达受到抑制;但在低浓度毒死蜱胁迫下,抗氧化酶活性并没有受到显著影响,而抗氧化酶基因的表达对毒死蜱更加敏感.毒死蜱能引 起 gstp2 的表达上调,但 GST 活性与 gstp2 的表达变化并不一致.处理组胚胎中 nrf2 表达上调,从而上调抗氧化蛋白和 II 相解毒酶基因的表 达.毒死蜱胁迫下,基因 ucp2 、cox1 表达下调,能够减少呼吸链 ROS 的产生.同时基因 bcl2 表达下调,表明凋亡的平衡受到破坏.毒死蜱处理 组中 MDA 含量显著升高,说明毒死蜱能造成斑马鱼胚胎氧化损 . 关键词：毒死蜱；斑马鱼胚胎；氧化应激；氧化损 中图分类号：X171.5 文献标识码：A 文章编号：1000-6923(2016)03-0927-08 Oxidative stress effects of chlorpyrifos on zebrafish embryos. ZHANG Jia-yu, LIU Li-li, LI Guo-chao, YU Kai-min, * LV Peng, YAN Yan-chun (Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China). China Environmental Science, 2016,36(3)：927~934 Abstract ：We elucidated the effects of chlorpyrifos exposure on the morphology, oxidative stress and oxidative damage in zebrafish (Danio rerio) embryos. After exposed to concentration gradient of chlorpyrifos solution for 96hours, the rates of deformities and death were dramatically higher than those in the control groups, and the median lethal concentration of CPF was calculated to be 1.18mg/L after a 96h exposure. The expression levels of oxidative stress-related genes, antioxidant enzyme activities and malondialdehyde (MDA) content were investigated. In CPF treatment groups, the antioxidant enzyme (SOD, CAT) activities and the expression levels of their encoding genes (Cu/Zn-sod, Mn-sod, cat) significantly decreased, compared with the control groups. At low concentrations of chlorpyrifo