睡眠剥夺对大鼠心电活动及血管内皮功能的影响 - 中国实验动物学报.doc

失眠增加心血管病致病因子的实验研究 袁蓉1,2，王阶，郭丽丽1，林飞1 （1.中国中医科学院广安门医院，北京 100053；2.北京中医药大学，北京 100029） 【摘要】目的：观察睡眠剥夺Sleep Deprivation (SD)对大鼠心血管病致病因子的影响，初步为中医安神治疗心血管病机制的研究提供基础实验依据。方法：16只大鼠随机均分为空白对照组Control Check (CC）和睡眠剥夺组(SD)。测量CC组及SD组大鼠SD前、SD后2天、5天、7天体重及心电图，并眼球取血测血清褪黑素（MT）、内皮素-1（ET-1）、炎症相关因子白介素6（IL-6）、肿瘤坏死因子（TNF-α）等。结果：与SD前比较，SD组大鼠体重明显减轻，CC组大鼠体重明显增加，SD后5天及7天，SD组大鼠体重与CC组比较明显减轻(P0.05)；与CC组比较，SD组大鼠SD后5天TNF-α升高，差异具有统计学意义（P0.05）；随SD时间的延长心率逐渐增快，QTc间期逐渐延长，MT逐渐降低，ET-1、IL-6、TNF-α逐渐升高，7天时两组的各指标差异均具有统计学意义（P0.05）。结论：长期失眠可使体重减轻，心率明显加快，QTc间期延长，褪黑素水平降低，内皮素和炎症因子升高，增加心血管病致病因子，为进一步研究安神中药对心血管病的作用提供基础实验支持。 【关键词】睡眠剥夺；冠心病；心率；内皮；炎症 Effect of insomnia on rat cardiovascular disease factors YUAN Rong1,,WANG Jie1*,GUO Li-li1,LIN Fei1 Guang`anmen Hospital, China Academy of Chinese Medical sciences,Beijing,100053,China; 2.Beijing University of Chinese Medicine,Beijing,100029,Chiina) Abstract：Objective：Investigate the effect of insomnia on cardiovascular disease factors and offer the experimental evidence for treating cardiovascular disease with traditional Chinese medicine tranquillization methods . Methods：Sixteen Wistar rats were randomly divided into two groups named sleep deprivation (SD) group and normal control check(CC) group. Body weight and electrocardiogram were recorded and serum concentrations of melatonin (MT), endothelin-1 (ET-1), IL-6 and TNF-α were tested before SD and 2d, 5d, 7d after SD. Results: Body weight decreased in SD group while increased in CC group. Compared with CC group, body weight of SD rats decreased significantly in 5d and 7d after SD(P0.05). Compared with CC group, TNF-α increased significantly in 5d(P0.05). With the time, heart rate accelerate and QTc were prolonged, MT decreased while ET-1, IL6, TNF-α increased significantly in 7d after SD(P0.05). Conclusion: Long term insomnia would decrease body weight and MT, while increase heart rate, QTc, ET-1 and inflammatory factors, which increase cardiovascular disease factors. It provided the experimental evidenc