三种药用真菌核苷类成分的分析及含量比较.docVIP

三种药用真菌核苷类成分比较，程敏2，鲁静3，王有为1 （1. 武汉大学药学院，湖北 武汉 430072；2. 武汉市药品检验所，湖北 武汉 430012；3. 中国药品生物制品检定所，北京 100050） [摘要] 目的：建立同时测定核苷类成分的定量分析方法，检测并比较核苷类成分的化学组成及其差异。方法：采用高效液相色谱法，Platinum C18分析柱，乙腈：水（5：95）为流动相，检测波长为260 nm，对猴头菌虫草乌灵参菌中核苷类成分进行分析。结果：鉴定了三种主要核苷，即腺苷、尿苷和鸟苷；方法学考证表明腺苷、尿苷和鸟苷的线性范围分别为2.51μg(mL-1~25.10 μg(mL-1、4.06μg(mL-1~40.60 μg(mL-13.48μg(mL-1~34.80 μg(mL-1，平均加样回收率分别为100.5 %、96.5 %和104.1 %，RSD分别为2.3%、1.0 %和3.9 %；不同真菌中核苷类成分的组成及含量有，其中在发酵虫草菌中三种主要核苷成分的含量最高虫草可检出虫草菌素和腺嘌呤，而猴头菌和乌灵参菌中则。结论：本文所建立的高效液相色谱法灵敏、快速、准确，可用于猴头菌等药用真菌中核苷类成分的分析；同时不同真菌中核苷成分的含量组成明显差异，可为不同药用真菌及其制定相应质量控制标准提供参考。 [关键词] 高效液相色谱法猴头菌；；核苷类etermination of nucleosides in several medicinal fungi by HPLC method Tang Jun1, Cheng Min2, Lu Jing3, Wang You-wei1 (1. College of Pharmacy Wuhan University, Wuhan 430072, China; 2. Wuhan Institute for the Drug Control, Wuhan 430012, China; National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China) ABSTRACT Objective: To establish a convenient HPLC method for both qualitative and quantitative analysis of nucleosides in Hericium erinaceus, the powdered fermentative hyphae of Cordyceps and Xylaria Fungi, meanwhile determine and compare the composition and quantitative differences of the nucleosides in these subjects. Methods: A reversed-phase HPLC method was applied for the analysis of nucleosides in Hericium erinaceus and the powdered fermentative hyphae of other medicinal fungi using a Platinum C18 column as stationary phase and acetonitrile-water (5: 95) as mobile phase, the flow rate was 1.0 mL·min-1; the system was operated at 22 (C; all nucleosides were detected at 260 nm. Results: Three major nucleosides (adenosine, uridine and guanosine) were identified and determined in Hericium erinaceus and the powdered fermentative hyphae of other medicinal fungi; method validation showed the linear ranges of adenosine, uridine and guanosine as 2.51μg(mL-1-25.10 μg(mL-1, 4.06μg(mL-1-40.60 μg(mL-1 and 3.48μg(mL-1-34.80 μg(mL-1, the mean recovery rate as 100