深黄被孢霉利用不同碳源产油脂比较.PDFVIP

生 物 工 程 学 报 Chin J Biotech 2010, February 25; 26(2): 189–193 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ ©2010 CJB, All rights reserved. 工业生物技术 深黄被孢霉利用不同碳源产油脂比较 1 1 1 1 2 邢大辉 ，潘安龙 ，薛冬桦 ，方鸣玉 ，谷冉峰 1 长春工业大学化学与生命科学学院，长春 130012 2 长春长生基因药业股份有限公司，长春 130012 摘 要: 本研究主要探讨深黄被孢霉M2 菌株对生物质全糖的利用，考察其碳源同化能力、不同碳源下产脂情况以及对 玉米皮渣的利用能力。研究结果表明，M2 菌株能够利用葡萄糖、木糖、阿拉伯糖和甘露糖进行生长和油脂积累。M2 菌株以6 ％糖浓度的玉米皮渣水解液为底物发酵培养，油脂微生物生物量达18.2 g/L，干菌体油脂含量45.7 ％，单位体 积发酵液油脂产量为8.3 g/L。 关键词: 深黄被孢霉，微生物油脂，生物质，玉米皮渣，发酵 Biomass carbohydrates assimilation and lipid accumulation by Mortierella isabellina 1 1 1 1 2 Dahui Xing , Anlong Pan , Donghua Xue , Mingyu Fang , and Ranfeng Gu 1 School of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China 2 Changchun Changsheng Gene Pharmaceutical Company Limited, Changchun 130012, China Abstract: Biomass carbohydrates assimilation and lipid accumulation by Mortierella isabellina M2 strain were investigated. Corn fiber hydrolysate was specially studied. The results showed M. isabellina M2 strain achieved growth and lipid accumulation while glucose, xylose, mannose and arabinose were introduced as single carbon source, respectively. When M. isabellina M2 strain was cultivated on corn fiber hydrolysate with 6% sugars concentration, the biomass reached 18.2 g/L, the lipid content of dry mycelia was 45.7%, and the lipid yield achieved 8.3 g/L. It provided a promising perspective for microbial oils production with biomass hydrolysates. Keywords: Mortierell