剩余污泥热碱解及其用于微生物油脂生产的探索.PDFVIP

生 物 工 程 学 报 Chin J Biotech 2011, March 25; 27(3): 482−488 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn ©2011 CJB, All rights reserved. 生物柴油与微生物油脂 剩余污泥热碱解及其用于微生物油脂生产的探索 1,2 2 1 2 徐静阳 ，大浦 宏隆 ，刘德华 ，梶原将 1 清华大学化学工程系应用化学研究所，北京 100084 2 日本东京工业大学生命理工学部，横滨 226-8501 摘 要: 为了将剩余污泥中的蛋白质成分进行资源化利用，采用热碱解法进行处理，并尝试将处理后得到的上清液作 为氮源培养圆红冬孢酵母用于微生物油脂的合成。结果表明，pH 13 下处理5 h 的条件对于污泥减量以及含氮物质析出 最为有效，而污泥在pH 10 处理5 h 时得到的上清液则相对更适于酵母培养。在剩余污泥中添加NaOH 调节初始pH 为 10，在60 ℃下处理5 h 后中和体系pH 值至7 ，得到的上清液经微孔膜过滤后用于配制限氮培养基可以使菌体生长良好， 并且细胞内油脂含量达35 ％。 关键词: 剩余污泥，热碱解，氮，产油酵母，微生物油脂 Heat-alkaline treatment of excess sludge and the potential use of hydrolysate as nitrogen source for microbial lipid production 1,2 2 1 2 Jingyang Xu , Takahiro Oura , Dehua Liu , and Susumu Kajiwara 1 Institute of Applied Chemistry , Department of Chemical Engineering , Tsinghua University, Beijing 100084, China 2 Department of Life Science , Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan Abstract: To recover and use protein resources in excess sludge and decrease the cost of microbial lipid production, heat-alkaline was used to treat the sludge, and the hydrolysate was preliminarily used as nitrogen source to cultivate Rhodosporidium toruloides AS 2.1389 for lipid accumulation. Firstly, we treated the excess sludge under different alkaline conditions (pH 10, pH 12, pH 13) within the reaction time of 5–10 h to investigate the effect of nitrogen source release. Secondly, we used the sludge hydol