青翘与老翘功效差异在经典方剂中的验证研究.pdfVIP

2017 年5月第 24卷第5期 中国中医药信息杂志 ·39· 论著 ·实验研究 青翘与老翘功效差异在经典方剂中的验证研究 孙迎娜，岳永花，刘聪，何盼，白关亚，倪艳 山西省中医药研究院，山西 太原 030012 摘要：目的 采用经典方剂连翘散和银翘散验证青翘和老翘的功效差异，为指导连翘“一体两用”提供实 验依据。方法 采用金黄色葡萄球菌制作豚鼠疮疡模型，将 40 只豚鼠随机分为空白组、模型组、连翘散青翘 组和连翘散老翘组，空白组和模型组灌服生理盐水，连翘散青翘组和连翘散老翘组灌服 1.2 g 原药材/kg 药液， 连续 7 d ，观察各组模型豚鼠疮疡症状积分、血常规及病理变化。采用 10%脂多糖雾化吸入法诱导大鼠急性肺 损伤模型，将 40 只 SD 大鼠随机分为空白组、模型组、银翘散青翘组和银翘散老翘组，空白组和模型组灌服 生理盐水，银翘散青翘组和银翘散老翘组灌服 4 g 原药材/kg 药液，连续 10 d，观察各组大鼠肺洗液白细胞介 素（IL ）-6、肿瘤坏死因子（TNF ）-α、IL-1β 水平。结果 连翘散青翘组抑制豚鼠疮疡伤口红肿作用较老翘组 起效快，但在抑制豚鼠疮疡伤口分泌物和病理改善方面与连翘散老翘组差异无统计学意义。银翘散青翘组大鼠 较老翘组 IL-6、TNF-α、IL-1β含量降低，但差异无统计学意义。结论 不同复方中青翘与老翘功效有一定差异。 关键词：青翘；老翘；连翘散；银翘散；疮疡模型；急性肺损伤模型；功效差异 DOI：10.3969/j.issn.1005-5304.2017.05.010 中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2017)05-0039-05 Validation Study on Efficacy Difference of Green Forsythiae Fructus and Ripe Forsythiae Fructus in the Classic Formula SUN Ying-na, YUE Yong-hua, LIU Cong, HE Pan, BAI Guan-ya, Ni Yan (Shanxi Institute of Traditional Chinese Medicine, Taiyuan 030012, China) Abstract: Objective To compare the efficacy difference of Green Forsythiae Fructus and Ripe Forsythiae Fructusby using Lianqiao powder and Yinqiao powder in the classic formula; To provide experimental evidence for the guidance of “one for dual-use” of the Forsythiae Fructus. Methods The guinea pig sore model was made with Staphylococcus aureus. 40 guinea pigs were randomly divided into blank group, model group, Lianqiao powder Green Forsythiae Fructus group and Lianqiao powder Ripe Forsythiae Fructus group. The blank group and the model group were fed with normal saline, while Lianqiao powder Green Forsythiae Fructus group and Lianqiao powder Ripe Forsythiae Fruct