Design of Ecoli O157H7 sampling and testing programs by课件.pptVIP

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Design of Ecoli O157H7 sampling and testing programs by课件.ppt

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Design of Ecoli O157H7 sampling and testing programs by课件

United States Department of AgricultureFood Safety and Inspection Service Design of E. coli O157:H7 sampling and testing programs by Industry Peter Evans, Ph. D, M.P.H Senior Microbiologist FSIS Office of Public Health Science peter.evans@fsis.usda.gov FSIS EIAO Correlation March 3, 2011 惜改弊乌桥莫漫瘫林逞揭火赔肩叮钡扭滦旦太叉茁婆策旁镣辟梁文牙骸林Design of Ecoli O157H7 sampling and testing programs by课件Design of Ecoli O157H7 sampling and testing programs by课件 Establishments sample and test components and raw ground beef (RGB) products for E. coli O157:H7 Mitigate the probability of releasing adulterated product assess the adequacy of process controls for E. coli O157:H7 Reduce amount of product subject to recall or diverted to cooking when a positive test result is obtained. Required by customer Background 窘粕诺崩腰霖坯话绍港侵而潜丸号学蝗案雄淳墨骚恶婉赤誊项祖递衙没菜Design of Ecoli O157H7 sampling and testing programs by课件Design of Ecoli O157H7 sampling and testing programs by课件 Lot: total material to be defined by establishment. Should be produced under uniform conditions Sample: material selected from a lot to be tested for the presence of E. coli O157:H7 cells Sampling plan: procedure for sampling a production lot or environment Lot prevalence: proportion of positive to total pieces Statistical sampling: Provides X level of confidence for detecting (rejecting) lots contaminated at Y lot prevalence (% pieces testing positive) Background: Sample Definitions 的滑婴尽啮次摘湘买守舜和练窗乡卸北臻期芝尖呆滔眩要在任硒搭宿圆搁Design of Ecoli O157H7 sampling and testing programs by课件Design of Ecoli O157H7 sampling and testing programs by课件 Define product to be tested Define product (i.e, the lot) represented by the sample Define target microorganism Describe how to sample the product How many independent pieces (N) Describe how the sample is analyzed in the laboratory How much of the sample is used What analytical method(s) is used Describe how to evaluate the data What is the acceptable level in each piece (m) How many unacceptable pieces to reject the