转基因相关药品配制.docVIP

转基因相关药品配制 stock solution ●MSmax stock solution（10×） NH4NO3 16.5g KNO3 19g KH2PO4 1.7g MgSO4·7H2O 3.7g CaCl2·2H2O 4.4g or CaCl2 3.32g Dissolve them one by one .Then add H2O to1000ml. ●MSmin stock solution (100×) KI 0.083g H3BO3 0.62g MnSO4·4H2O 2.23g or MnSO4·H2O 1.69g ZnSO4·7H2O 0.86g Na2MoO4·2H2O 0.025g CuSO4·5H2O 0.0025g CoCl2·6H2O 0.0025g Note：Na2MoO4 must be dissolve independently and then mix with the other composition .Add H2O to 1000ml and store at room temperature. ●N6max stock solution（10×） KNO3 28.3g KH2PO4 4g (NH4)2SO4 4.63g MgSO4·7H2O 1.85g CaCl2·2H2O 1.66g or CaCl2 1.25g Dissolve them one by one .Then add H2O to1000ml and store at room temperature. ●N6min stock solution (100×) KI 0.08g H3BO3 0.16g MnSO4·4H2O 0.44g or MnSO4·H2O 0.3335g ZnSO4·7H2O 0.15g Add H2O to1000ml and store at room temperature. ●Fe2+–EDTA stock solution (100×) 2.78g FeSO4·7H2O in a bottle containing 300ml H2O， 3.73g Na2EDTA·H2O in anther bottle containing 300ml H2O and heat to 70℃， after they both dissolved，mix together and keep at 70℃ for 2 hours, then add H2O to1000ml and store at 4℃. ●Vitamin stock solution (100×) Nicotinic 0.1g Pyridoxine HCl（VB6） 0.1g Thiamine HC l（VB1） 0.1g Glycine 0.2g Inositol 10g Add H2O to1000ml and store at 4℃. ●AAmax stock solution（10×） KCl 29.5g NaH2PO4 1.5g or NaH2PO4·2H2O 1.95g MgSO4·7H2O 2.5g CaCl2·2H2O 1.5g or CaCl2 1.13g Add H2O to1000ml and store at room temperature. ●AAmin stock solution (100×) MnSO4·H2O 1g H3BO3 0.3g ZnSO4·7H2O 0.2g KI 0