实验三 食品中蛋白质含量测定(凯氏定氮法)（Determination of protein content in three foods (kjeldahl method)）.docVIP

实验三 食品中蛋白质含量测定(凯氏定氮法)（Determination of protein content in three foods (kjeldahl method)） Determination of protein content in three foods (kjeldahl method) (determination of protein content in 5009.5-2003 food) I. purpose and requirements 1. Learn the principle of determination of protein by kjeldahl. 2. Mastered the operation technology of kjeldahl, including the digestion, distillation, titration and protein content of the samples. 2. Experimental principle Protein is a nitrogen-containing compound. Food together with concentrated sulfuric acid and catalyst heating digestion, decompose protein, produced by ammonia and sulfuric acid combined with ammonium sulfate, in digestive juices, and alkaline distilling ammonia dissociation, with boric acid after absorption, again with hydrochloric acid standard solution titration, according to the consumption of acid conversion factor times the protein, a quick protein content. This protein is called crude protein because it contains other nitrogen-containing substances besides protein. Iii. Instruments and reagents (1) reagent 1. Copper sulfate (CuSO4 · 5H20) 2. Potassium sulfate 3. Sulfuric acid (1.8419 g/L) 4. Boric acid solution (20g/L) 5. Sodium hydroxide solution (400g/L) 6. 0.01 mol/L hydrochloric acid standard titration solution. 7. Mixing indicator reagent: 0.1 methyl red ethyl solution liquid 1, mixed with 0.5 of the 0.1% bromophenol green ethanol solution. 8, soybean meal. (2) instruments Trace determination of nitrogen distillation apparatus: as shown in FIG. 3 -. Figure 3 - trace kjeldahl apparatus 1. Electric furnace; 2. Steam generator (2L flat flask); 3. Spiral clip a; 4. Small funnel and rod glass plug (sample entrance); 5. Reaction room; 6. Reaction outdoor layer; 7. Rubber tubing and spiral clip b; 8. Condensing tube; 9. The distillate receives the bottle. 4. Experimental steps 1. Sample digestion Weigh and soybean powder 0.3 g (+ / - 0.001 g), into the dry 100 ml kjeldahl flask, add 0.2 g potassium sulfate,