黄连温胆汤对2型糖尿病大鼠糖脂代谢紊乱的改善作用 - 药学与临床研究.doc

黄连温胆汤2型糖尿病大鼠糖脂代谢紊乱 李月碧1，李瑞鹏1，赵迪1，杨京1，马世平1*（1中国药科大学中药药理学教研室，南京 211198；2南京中医药大学，南京 210046） 教育部博士点基金20113237120007），江苏省中医药局科技项目LZ13001)，江苏省高校自然科学研究计划（12KJD360002）zwhtcm@（第二通讯作者） 摘要 目的 考察黄连温胆汤2型糖尿病引起大鼠糖代谢紊乱作用。方法 通过60天高糖高脂饲料喂养合并一次性35mg/kg 链脲佐霉素（STZ）腹腔注射构建2型糖尿病大鼠模型测定血清中空腹血糖（FBG）血清胰岛素（FINS）胰岛素敏感系数（ISI）胰岛素抵抗系数（IRI）黄连温胆汤对大鼠糖代谢紊乱的。结果 黄连温胆汤能降低FBG，GHB，增加ISI，降低IRI；降低血清TG，CHO和HDLC含量，升高HDLC含量。结论 黄连温胆汤可以有效地改善2型糖尿病引起的糖脂代谢紊乱。 关键词 代谢紊乱 糖尿病 Protective Effect of Huanglian Wendan Decoction on Metabolism Disorder in a Rat Model of Type 2 Diabetic Mellitus Abstract Objective: The aim of this study is to investigate the therapeutical effect of Huanglian Wendan decoction on metabolism disorder in rat model of type 2 diabetic mellitus. Methods: 60 days high-glucose-high-fat diet and 35 mg/kg streptozocin (STZ) injection once were used to bulid a rat model of type 2 diabetic mellitus.Following, metformin hydrochloride (0.2 g/kg), berberine hydrochloride (0.2 g/kg), HLWDD(3, 6, 12 g/kg) were given by gavage for 30 days except normal and model groups after randomly divided into six groups. Relate measurements about glucose and lipid metabolism in serum were did, and insulin sensitivity index (ISI) and insulin resistance index (IRI) were calculated to evaluate the improvement of Huanglian Wendan decoction on metabolism disorder. The pathological damage of pancreas was observed by a HE staining. Result: 30 days administration of Huanglian Wendan decoction to the rats reduced fast blood glucose (FBG), glycosylated hemoglobin(GHB), IRI, triglyceride (TG), total cholesterol (CHO) and low density lipoprotein cholesterol (LDLC) in serum; increased ISI and high density lipoprotein cholesterol (HDLC). However, its effect to FINS can not significantly observed. Treatment of HLWDD to rats also protected them from pathological damage of pancreas. Conclusion: Treatment of Huanglian Wendan decoction protects rats from metabolism disorder and pathological damage of pancreas in a rat model of type 2 diabetic