哺乳动物非经典分泌信号肽介导重组EGFP蛋白向毕赤酵母.PDFVIP

生 物 工 程 学 报 覃玉凤 等/ 哺乳动物非经典分泌信号肽介导重组EGFP 蛋白向毕赤酵母细胞壁转运 1455 Chinese Journal of Biotechnology /cjbcn October 25, 2016, 32(10): 1455−1464 DOI: 10.13345/j.cjb.160007 ©2016 Chin J Biotech, All rights reserved 生物技术与方法 EGFP 覃玉凤，陈瑶生，刘志国，张英，刘海龙，何祖勇 510006 , , , . EGFP . , 2016, 32(10): 1455–1464. Qin YF, Chen YS, Liu ZG, et al. Delivery of recombinant enhanced green fluorescent protein to Pichia pastoris cell wall directed by a mammalian nonclassical secretion signal peptide. Chin J Biotech, 2016, 32(10): 1455–1464. 摘 要: 为探索哺乳动物非经典分泌信号肽在毕赤酵母表达系统中引导重组蛋白分泌的作用，本研究将一段 来源于小鼠同源异型框蛋白 (En2) 的分泌信号序列 (SS) 融合至EGFP 蛋白的N 端，在毕赤酵母中表达。实 验结果显示SS 信号肽能通过一种不同于经典的内质网- 高尔基体分泌通路的方式将EGFP 蛋白分泌至细胞膜表 面，与α 交配因子前导肽相比，显著降低了细胞的内质网压力。本研究提示哺乳动物非经典分泌信号肽可作为 递送重组蛋白至酵母膜表面的一项工具。 : 内质网压力，非经典分泌，毕赤酵母，qPCR Delivery of recombinant enhanced green fluorescent protein to Pichia pastoris cell wall directed by a mammalian nonclassical secretion signal peptide Yufeng Qin, Yaosheng Chen, Zhiguo Liu, Ying Zhang, Hailong Liu, and Zuyong He State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, Guangdong, China Abstract: A mammalian nonclassical secretion sequence derived from mouse Engrailed2 homeoprotein (En2) was used to direct the secretion of the enhanced green fluorescent protein from Pichia pastoris . This signal peptide conferred the Received: January 6, 2016; Accepted: March 9, 2016 Supported by: National Transgenic Major Program (No. 2016Z006), Natural Science Foundation of Guangdong Province (No. 2016A030313310). Corresponding author: Zuyong He. Tel/Fax: +86-20 E-mail: zuyonghe@ (No. 2016Z006)