淮海工学院（Huaihai Institute of Technology）.docVIP

淮海工学院（Huaihai Institute of Technology） Collected by oneself Mistakes are unavoidable For reference only In case of error Please correct me! Thank you The role of high performance liquid chromatography in food analysis 1. Preface The trend of modern analytical instruments is high speed High accuracy high sensitivity Effort saving and automation In the field of chromatography From the second half of the last century of 60s The wide application of gas chromatography has made the column chromatography develop dramatically The methods and techniques developed in column chromatography are also used in thin layer chromatography and liquid chromatography Thus, the function of the chromatography is greatly improved The application field is also expanding To distinguish these modern chromatography from the old methods They are called high-performance chromatography Establishment of high performance chromatography The position of chromatography in analytical chemistry has been improved nowadays Chromatography is used in the analysis of complex substances and mixtures of components It is a very important method of analysis The purpose of the application of chromatography is to make quantitative analysis and separate pure substances Actually According to the purpose of analysis One or several of them used in combination with gas chromatography, liquid chromatography, and thin-layer chromatography In liquid chromatography and thin layer chromatography All substances soluble in mobile phase can be used as the object of analysis Because liquid chromatography is highly effective, simple and fast, it is highly recommended by analysts Therefore, the use of more extensive Thin layer chromatography has long analysis time The quantitative accuracy differences as HPLC pre experimental method High performance liquid chromatography (HPLC) is a separation and analysis technique developed in the late 60s It is an important means of modern separation and determination It is based on the classi