HeNe激光对兔眼小梁切除术后滤过道瘢痕化的影响及其机制.doc

　　HeNe激光对兔眼小梁切除术后滤过道瘢痕化的影响及其机制 ：王瑞琳,李贵刚,陈志祺,向艳,张虹 【摘要】 目的：研究HeNe激光对兔眼小梁切除术后滤过道瘢痕化的影响及其机制，探讨HeNe激光在青光眼小梁切除术中的应用价值。　　方法：于小梁切除手术前采用功率密度200mATERIALS AND METHODS 　　Materials 　　Experimental animal Tit, provided by the Experimental Animal Center of Tongji Medical College. 　　Instruments and reagents The HeNe laser machine used in this study anufactured by asson staining kit (ethods 　　Laser irradiation Site: the upper nasal limbus area next to the upper rectus muscle; diameter: 4mm; time: 10 minutes×3 days. 　　Surgery method Rabbits y: made a conjunctival flap based on fornix zone just next to the upper rectus muscle, and a 3mm×3mm scleral flap (2/3 sclera thickness). Excised 1.5mm×1mm trabecula tissue in the corneoscleral limbus. Cut a 1.5mm×1.5mm iris periphery hole. Reset the sclera flap, sutured the sclera flap and bulbar conjunctiva. 　　Animal grouping Tly divided into three groups, representing the 7th, 14th and 28th day after surgery. The right eyes received laser irradiation, the left eyes served as control. Seven eyes each group at each time point. 　　Observation and sample preparation assons staining e light intensity to observe the blue stained area and image analysis softinosity. 　　CTGF immunohistochemistry staining ark the antigen (CTGF) and observe after DAB staining. Result criterion: the CTGF staining positive cell . Five high poly in every slice, ber of fibroblast, and calculated the average positive cell percentage. 　　Statistical Analysis The SPSS (16.0) softed the sign for diversity had statistical significance. 　 　RESULTS 　　ia uch lesser and filtration bubble uch more obvious in laser group than those of control group. 　　The expression of connective tissue groall amount on the 28th day (Figure 1). The expression of laser group uch lesser than that of control group on the 7th and 14th day (t=3.078, 3.458; P=0.01, 0.005 ), and the expression diversity betage analysis shoportant, suppress the scar formation of filtration canal 　　Table 1 HeNe laser im