大肠癌中Ezrin蛋白的表达及其与临床病理特征的关系.doc

　　大肠癌中Ezrin蛋白的表达及其与临床病理特征的关系 ：范婕　史威 李秀娟 郭颖 白美玲 【摘要】 目的：探讨Ezrin在大肠癌中的表达特点及其与临床病理参数的关系。方法：应用免疫组织化学SP法检测100例大肠癌组织及11例癌旁正常黏膜中Ezrin的表达情况。结果：Ezrin阳性表达率在大肠正常粘膜、大肠癌组织中分别为36.4%、75%,差异有统计学意义(Plt;0.05);淋巴结转移组Ezrin阳性率(89.6%)显著高于无淋巴结转移组(61.5%)(Plt;0.05);在大肠癌中,Ezrin异常表达与大肠癌患者的年龄、性别、肿瘤的发生部位以及分化程度无关(Pgt;0.05)。结论：Ezrin表达水平提高提示癌组织具有更高的浸润、侵袭能力和淋巴结转移风险，检测 Ezrin有望成为判断大肠癌患者预后的新指标或 治疗 肿瘤转移的一个靶点。 【关键词】 肠肿瘤;肿瘤转移;免疫组织化学;膜细胞骨架连接蛋白 Ezrin,a member of the ezrinradixinmoesin(ERM)family in speciesconserved protein,is a membrane cytoskeleton linker and is involved in cellular functions,including epithelial cell morphogenesis and adhesion[1].It is encode by vil2(6q25.2～q26)and its functional domain is FERM domain.The inactivation of Ezrin causes a massive cell retraction and leads to the destruction of both cellcell and cellsubstrate adhesion,a munohistochemistry to elucidate its functional importance and clinical significance. 　　 1 Materials and methods 　　1.1 Materials 　　Beta samples the archives of the First Affiliated Hospital,Hebei North University Zhangjiakou,otherapy and radiotherapy before operation.There en(41%)and 59 en(59%)edian age of 58.3 years(range,30～78 years).Fortysix patients ors and fiftyfour patients in rectum.Fortyeight patients(48%)had histologically confirmed lymph node metastasis,aining 52 patients(52%)ph node involvement.According to current ethods 　　The samples alin and paraffin embedded,processed and stained p;E sections the diagosis.One paraffin block aximum bulk of tumor each case for immunohistochemical studies.All slides shoa junction.Serial sections of 4 μm thickness the paraffin blocks.The sections atic antigen retrieval ed on each slide and in.Sections in in 3% hydrogen peroxide to quench endogenous tissue peroxidase.The sections munostained for mono clonal antibody against Ezrin(mouse mAbIgG1,3C12;Lab Vision Neomarker Corp)at a dilution of 1:50 and incubated at 4 ℃ ily proteins.After in.And also after in.Then,DAB munohistochemical staining 　　His