镧示踪观察兔视网膜冲击伤后血视网膜屏障改变（Observation of changes of blood retinal barrier after retinal blast injury in rabbits by lanthanum tracer）.docVIP

镧示踪观察兔视网膜冲击伤后血视网膜屏障改变（Observation of changes of blood retinal barrier after retinal blast injury in rabbits by lanthanum tracer） Observation of changes of blood retinal barrier after retinal blast injury in rabbits by lanthanum tracer Update: 2011-09-15 nest Yang Zhengguo Yannian Hui Yang Zhihuan Yuan Jian Retinal damage is directly related to the impairment of the (blood-retinal, barrier, BRB) [1, 2]. The morphological changes of BRB after blast injury were observed by lanthanum tracer, which provided a theoretical basis for clinical practice. Materials and methods 1. groups: healthy pigmented rabbits 24, weight 2 ~ 2.5 kg, both male and female, were randomly divided into 6 groups, 1 hours after injury, 3 hours, 1 days, 3 days, 7 days and 14 days each time point were killed 4, making the light and electron microscope. 5 eyes without injury were served as normal control group. 2. animal model: Animal Anesthesia, using type BST bio shock tube double membrane pressure breaking membrane injury of rabbit unilateral positive time, peak pressure (Pm): (666 + 10.5) kPa, positive pressure duration (Tm): (7.25 + 0.96) Ms. Observation of 3.BRB lanthanum nitrate tracing electron microscope: injury at different time points after bilateral carotid artery intubation, simultaneous injection of lanthanum nitrate solution 20 ml, within 3 minutes of removal of the eye, eye after the concentration of glutaraldehyde solution in section 30 of the g/L in 20 minutes, in the dissection of choroid and retina tissue under the microscope, cut into 1 mm * 5 mm block, at the concentration of glutaraldehyde fixed 2 hours continue to 30 g/L, with three times two methyl 0.1 mol/L sodium citrate buffer rinse, then according to the conventional TEM specimen preparation procedures, ultrathin sections, observe and photograph by JEN 100SX electron microscope. The normal control group consisted of non injured eyes injected with tracer, and the method of electron microscope preparation was the same as ab