超高效液相色谱法测定烟叶中的茄尼醇含量.PDFVIP

80 Chinese Tobacco Science 2012-12 ，33 （6 ） 1 2 1 1 1 1 1* 1. 550081 2. 650091 UPLC 10 min1.7 μm BEH C18 5 min -Q-TOF-MSm/z 613.57 631.58 0.765~76.5 μg/mL 0.99924 7.65 ng/mLRSD 2.62%91.1% - S572.03 1007-5119201206-0080-05 DOI 10.3969/j.issn.1007-5119.2012.06.016 Determination of Solanesol Content in Tobacco Leaves by Using Ultra-high Pressure Liquid Chromatography 1 2 1 1 1 1 ZHANG Jie , WANG Yunsong , CAI Kai , XIANG Zhangmin , ZHOU Shuping , XU Dongqing , 1* GENG Zhaoliang (1. Guizhou Tobacco Research Institute, Guiyang 550081, China; 2. Yunnan University, Kunming 650091, China) Abstract ：In order to determine the content of free solanesol in tobacco leaves quickly and efficiently, a rapid Ultra-high Pressure Liquid Chromatography (UPLC) method was developed. By ultrasonic extraction with methanol, the extracting solution was membrane filtered to analyze by UPLC. The extract was eluted with methanol onto a short 1.7 μm BEH C18 column, and solanesol was separated rapidly in 5 min. According to the ion peaks of m/z 613.57 and 631.58, qualitative analysis was carried out by Electrospray Ionization-Quadropole Time-of-Flight Mass Spectrometry (Q-TOF-MS). The results showed that the linearity range of the proposed method was 0.765-76.5 μg/mL, the RSD% was 2.62%, and the average recovery rate was 91.1%. The method was appropriate for quick and sensitive determination of free solanesol in tobacco. Keywords: solanesol; tobacco leaf; UPLC; Q-TOF-MS C45H74O 1956