乐果降解菌的分离,筛选和鉴定.docVIP

审稿费到，已核稿，形式审查通过 基金项目：福建省自然科学基金“阴沟肠杆菌降解有机磷农药机理研究”（2006J0321）。 第一作者简介：马丽娜，女，1974年出生，福建福州人，助理研究员，学士，从事农药残留检测与降解研究，通信地址：350003 福州市五四路247号，福建省农科院农业生物资源研究所。Tel：0591E-mail：laeptb@163.com。 通讯作者：刘波，男，1957年生，福建惠安人，研究员，博士，主要从事生物技术和生物防治研究。通信地址：350003 福州市五四路247号，福建省农业科学院。Tel：0591E-mail：laeptb@163.com。 收稿日期:2008-04-07，修回日期：2008-0-08。 乐果降解菌的分离、筛选和鉴定 马丽娜1，官雪芳2，朱育菁1，林抗美1，刘 波1 （1福建省农科院农业生物资源研究所，福州 350003；2福建省农科院农业工程技术研究所，福州350003） 摘 要：筛选高效的有机磷农药乐果的降解菌。采集福建省福农生化有限公司排污口，沟口及草坪土壤进行乐果降解菌的分离鉴定。经含乐果的培养基分离培养、脂肪酸鉴定，总共得到17个属23株细菌，对这些菌通过消解试验进行复筛，得到4株对乐果有较好降解能力的菌株，沙门氏菌、阴沟肠杆菌、铜绿假单胞菌和枯草芽孢杆菌，在乐果浓度为1000mg/L时，48h的降解率分别为27.3%、26.6%、22.9%和18.1%。对乐果有降解作用的菌株存在种类多样性的特点。 关键词：乐果；降解菌；阴沟肠杆菌；沙门氏菌；铜绿假单胞菌；枯草芽孢杆菌 中图分类号：S481.8 文献标识码：A Isolation, Screening and Identification of Dimethoate-degrading bacteria Ma Lina1, Guan Xuefang, Zhu Yujing1, Lin Kangmei1, Liu Bo1 (1Institute of Agro-biological resources, Fujian Academy of Agricultural Sciences, Fuzhou 350003; 2Institute of Agricultural Engineering Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003) Abstract: To screen high-effective bacteria for Dimethoate degradation. 11 soil samples on of sewage outfall and surrounding land in Fujian Fu-nong Biochemistry Co., Ltd were collected to isolate the Dimethoate-degrading bacteria.23 bacterial stains were isolated by using the cultural media containing Dimethoate and identified by fatty-acid analysis. The bacteria were divided into 17 genera. Further screening was conducted by digestion test and 4 isolates were found to have good degrading ability to Dimethoate. Salmonella choleraesuis subsp. houtenae, Enterobacter cloacae, Pseudomonas aeruginosa and Bacillus megaterium GC subgroup A had 27.3%, 26.6%, 22.9% and 18.1% of degrading rate at 48h, respectively, while the initial concentration of Dimethoate was 1000 mg/L. The bacteria that had degrading ability to Dimethoate existed species polymorphism Keywords: dimethoate, degrading bacterium, Salmonella choleraesuis, Enterobacter