大熊猫核糖体蛋白亚基RPS7基因的克隆及序列分析.docVIP

大熊猫核糖体蛋白亚基RPS7基因 的克隆及序列分析 侯怡铃，侯万儒，郝彦哲，张 田 (西华师范大学生命科学学院，南充 637002) 摘要:关键词: 大熊猫RT-PCR； RPS7；克隆序列分析cDNA Cloning and Sequence Analysis of ribosomal protein S7 gene（RPS7）from Giant Panda（Ailuropoda melanoleuca） HOU Yiling, HOU Wanru*,HAO Yanzhe, ZHANG Tian(College of Life Science, China West Normal University, Nanchong 637002) 　Abstract: Understand the structure of ribosomal protein S7 (RPS7) gene for giant panda (Ailuropoda melanoleuca) and the homologies with other already reported, including Homo sapiens, Mus musculus, Rattus norvegicus and Bos taurus. Methods: The cDNA of RPS7 was cloned from the Giant Panda (Ailuropoda melanoleuca) using RT-PCR technology; The sequence data were analyzed by GenScan software; Homology research of the Giant Panda RPS7 compared with the gene sequences of other species ware performed using Blast 2.1; ORF of the DNA sequence was searched using ORF finder software ; Protein structure of the RPS7 sequence cloned was deduced using PredictProtein software. Results: The full length of the expression sequence was 589 bp containing an ORF of 585 bp. The deduced protein sequence showed that the protein was composed of 194 amino acids and its estimated molecular weight was 22.12685 kDa with a pI of 10. 09. There were 7 different patterns of functional sites: one N-glycosylation site; two cAMP and cGMP-dependent kinase phosphorylation sites; four casein kinase C phosphorylation sites; one Casein kinase II phosphorylation site; two N-myristoylation sites; two Amidation sites and one Ribosomal protein S7e signature site in the RPS7 protein. Further analysis indicated that the expression sequence of RPS7 and the protein encoded were highly homologous to some mammals reported. Conclusion: The complete coding sequence of RPS7 gene has been cloned through RT-PCR technique successfully, which is the first report on the RPS7 gene from the Giant Panda. The data will not only enrich and supplement the information about RPS7 but also allow the isolati