丹参酮IIA增效大鼠骨髓源性内皮祖细胞VEGF,SDF-1表达.docVIP

丹参酮IIA增效大鼠骨髓源性内皮祖细胞VEGF、SDF-1表达 杨彦 陈庆伟 曹广煜 李桂琼 （重庆医科大学附属第二医院老年心血管病科，重庆 400010） 【摘要】 目的 研究在丹参酮IIA的辅助下，骨髓源性内皮祖细胞（EPCs）VEGF与SDF-1表达是否能增强。方法 Percoll密度梯度离心法分离SD大鼠骨髓单个核细胞，血管内皮生长因子（VEGF）、表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)诱导培养，并进行形态学、免疫学、功能学（Dil-acLDL与FITC-UEA-1双荧光染色）R541.4 【文献标志码】A Efficiency of rat bone marrow-derived endothelial progenitor cells on VEGF and SDF-1 expression with the aid of Tanshinone IIA . Yang Yan, Chen Qing Wei*, Cao Guang Yu, Li Gui Qiong. Department of Gerontology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. 【Abstract】Objective To investigate the effect of rat bone marrow-derived endothelial progenitor cells on VEGF expression with the aid of Tanshinone IIA. Methods The mononuclear cells were isolated from rat femoral and tibial bone marrow using percoll density gradient centrifugation, then induced with vascular endothelial growth factor(VEGF)、basic fibroblast growth factor(bFGF) and epidermal growth factor (EGF)for two weeks. The expression of cell markers was assessed by immunocytochemistry, and the attached cells were stained with Dil-acLDL and FITC-UEA-1. Then this study included four groups: Tanshinone IIA, EPCs, EPCs+ Tanshinone IIA and control groups. The expression levels of VEGF and SDF-1 was detected by fluorescent qRT-PCR and western blot．Results Compared with the other three groups, EPCs+ Tanshinone IIA group had a higher VEGF and SDF-1 expression（P＜0.05）. Conclusions EPCs can increase VEGF expression with combination of Tanshinone IIA. Homing rate and repairing efficacy of EPCs can improve also with the aid of Tanshinone IIA. EPCs can increase VEGF and SDF-1 expression in injured myocardium after homing, thus improve angiogenesis efficiently. 【Key words】bone marrow；endothelial progenitor cells; Tanshinone IIA; angiogenesis Supported by the General Program of National Natural Science Foundation of China (No Corresponding author: Chen Qing Wei, Tel Email: chenqwc