Thrsp在不同乳品质奶牛乳腺中的表达及其对乳脂合成的影响.pdfVIP

v，Ⅵnv．chinadairy．net 中国乳品工业 zgrpgy@163．corn Thrsp 腺中的表达及其对乳脂合成的影响 刘智宇，李庆章，高学军，崔英俊 (东北农业大学于。-on科学教育部重点实验室，哈尔滨150030) Hormone 摘要：利用数字基因表达谱及荧光定量PCR对不同乳品质奶牛及干奶期奶牛乳腺甲状腺激素应答蛋白(ThyroidRespon— sive 14 Spotprotein，THRSP)基因Thrsp 后转染奶牛乳腺上皮细胞，检测Thrsp过表达对奶牛乳腺上皮细胞乳脂合成的影响。结果表明：泌乳期高乳品质奶牛ThrspmRNA表 关键词：ThrSp；奶牛；乳腺上皮细胞；乳脂 中图分类号：TS252．1 文献标识码：A 文章编号：1001—2230(2014)09—0019—03 indifferentmilk COW andit’S Thrspexpression qualitydairy mammarygland onmilkfat effect synthesis LIu Qing—zhang，GAOXue-jon，CUI Zhi—yu，LI Ying-jun of Scienceof ofEducation，Northeast (KeyLaboratoryDairy Ministry AgriculturalUniversity，Harbin 150030，China) in Abstract：Differentialof inlactationand COW were DGEand expressionThrsp dry dairy mammarygland qRT—PCR． period analyzedby of COW cellswas of built．ThenCOW cells mammary 确砷genedairy epithelial cloned．Expressionplasmidn唧was dairy mammaryepithelial of weretransfacted．Theeffects of onmilkfat in COW ceUswere over—expression mammary Thrsp synthesisdairy epithelial showedthat mRNA inthe milk dainrCOWwas thanthatinlowmilk Thrsp expressionhigh quality higher qualitydairy concentrationin COW cellswasincreasedafter of glycefide dairy mammaryepithelial over—expressionThrsp wasinvolvedinthemilkfat in COW． synthesisdairy fat