天狼猩红染色法在肌腱胶原形态分析中的应用.docVIP

天狼猩红染色法在肌腱胶原形态分析中的应用 王继宏（硕士） 温树正（学士） （作者单位：内蒙古医学院第二附属医院手显二科） 通讯作者：温树正（nmgwsz@，电话真：0471－6351329） （通讯地址：呼和浩特市内蒙古医学院第二附属医院手显二科 010030） 正文字数（不计空格）：3092字；表数：2；图数：16 [摘要] 目的 观察和探索天狼猩红染色法在肌腱胶原形态分析研究中的应用价值。方法 将30只10月龄雄性leghorn鸡随机分为A、B、C 组，每组10只。将每只鸡的左足第三趾造成挤压撕脱伤模型，用改良Kessler缝合法缝接。A组、B组、C组分别于术后2周、4周、8周取材,对标本进行磷钨酸苏木素染色、天狼猩红染色、免疫组化染色等观察。结果 A组腱缝合段内的胶原纤维数量少, 排列稀疏，以纤细的Ⅲ型胶原为主； B组腱缝合段内的胶原纤维数量较多, 排列紊乱，Ⅰ、Ⅲ型胶原交错排列； C组腱缝合段内的胶原纤维数量多,以粗大的Ⅰ型胶原为主；成纤维细胞数量少，腱细胞成熟。结论 1、天狼猩红染色法在胶原纤维的染色中可在同一张切片中同时显示Ⅰ型和Ⅲ型胶原，是研究肌腱损伤修复中胶原纤维形态变化的重要染色方法。2、肌腱在愈合早期以纤细的Ⅲ型胶原为主，愈合晚期则以粗大的Ⅰ型胶原为主。 【关键词】肌腱损伤 修复 天狼猩红染色 实验研究 Sirius red staining in the study of tendon repair Wang ji-hong, Wen Shu-zheng,et al. Hand microsurgery Department Ⅱ，The Second Affiliated hospital of Inner Mongolia Medical college， Inner Mongolia, Hohhot 010030, China Corresponding author: Wen Shu-zheng Email:nmgwsz@ Objective Observe and explore the sirius red stain in the study of the role of tendon repair. Methods　 Thirty ten-month old fowl were randomly divided into groups A,B and C (10 each group).Cut off the flexor tendon in the third toe then suture it . A group, B group, C group, respectively after 2 weeks, 4 weeks, 8weeks drawn and did the specimens biomechanical test, sirius red staining, immunohistochemical staining, the specimens were evaluated by means of macroscopic observation. Results A group within the section of tendon suture a small number of collagen fibers, arranged in sparse, thin collagen type Ⅲ to the main; B group within the section of tendon suture collagen fibers are more disordered, Ⅰ, Ⅲ collagen staggered; C group tendon suture paragraph quantity of collagen fibers, with thick main type Ⅰ collagen; a small number of fibroblasts, tendon cell maturation. Conclusion 1, Sirius red staining of collagen fibers can be dyed simultaneously in the same slide show type Ⅰ and Ⅲ collagen, is to study the tendon collagen fibers in the repair of major staining. 2, tendon healing in the early to slim type Ⅲ collagen-base