2型糖尿病prckz基因的高甲基化研究.doc

2型糖尿病PRCKZ基因的高甲基化研究【摘要】 探讨PRKCZ基因的高甲基化变化与2型糖尿病的相关性。采用病例-对照方法研究272例十堰市太和医院无亲缘关系个体，分为两组： 2型糖尿病组T2DM组、正常对照组NC组（）甲基化芯片（）甲基化硫化测序PCR（bisulfite sequencing PCR, BSP）及测序来分析PRKCZ基因启动子甲基化状态与2型糖尿病的相关性；（）酶联免疫吸附实验（ELlSA检测血清PRKCZ蛋白水平。采用SPSS17.0统计分析软件进行。与正常对照组相比，2型糖尿病组的PRKCZ基因启动子，9个CpG位点有6个显示发生了甲基化，正常对照组1个显示发生了甲基化2型糖尿病组和对照组CpG位点甲基化的具有极显著性差异（p0.01）2型糖尿病组，PRKCZ蛋白表达降低，差异有统计学意义（p0.05）结论 PRKCZ基因启动子的高甲基化可能参与2型糖尿病的发生发展。 【】 2型糖尿病PRCKZ基因基因芯片高甲基化表观遗传学 【】【】 Hypermethylation of PRKCZ in type 2 diabetes mellitus ZOU Li1,YAN Shirong2 (1.Gu Cheng People Hospital Of Hu Bei,Gu Cheng, 441700;2.Hubei Medical College,Shi Yan ,44200) 【bstract】 Objective relationship between PRKCZ gene promoter methylation state and type 2 diabetes mellitus。ethods A case-control study included 272 participant who were not family related with each other and was performed in the Taihe hospital of Shiyan City. All participants were divided into two groups: T2DM group (type 2 diabetes mellitus) that contained 152 patients, and NC group (normal control) that included 120 volunteers. Both NimbleGen MeDIP-chip and bisulfite sequencing PCR(BSP) were utilized to analyze the methylation level of PRKCZ promoter. ELlSA was used to examine the serum level of PRKCZ. Statistical analyses were performed with SPSS17.0 software. Results We found the methylation level of the PRKCZ gene promoter of T2DM was higher than that of NC group. Six CpG sites of nine CpG sites on the PRKCZ gene promoter were proved to be Methylated in T2D group, whereas only one CpG site was tested to be Methylated in NC group. And the difference of methylation between these two groups was statistical significant (p0.01). Furthermore, the serum level of PRKCZ in T2DM was decreased as compared to that in NC group (P0.01). Conclusion The hypermethylation state of PRKCZ gene promoter is obviously higher in T2DM than NC group, suggesting that PRKCZ gene promoter hypermethylation may be involved in the pathogenesis of T2DM. 【Key words】 T2DMPRCKZ gene; gene chip;