多巴胺d1d3受体敲除及双基因敲除对小鼠体重-中国试验动物学报.docVIP

研究报告 多巴胺D1、D3受体及双敲除小鼠 张宝，刑博，魏曙光，，李生斌（西安交通大学医学卫生部法医学重点实验室环境与疾病相关基因教育部重点实验室，陕西西安 710061） 研究多巴胺受体介导的神经活动方法 引进多巴胺D1和D3受体敲除小鼠，繁育出多巴胺D1D3受体双敲除小鼠。探讨多巴胺D1受体、D3受体D1D3受体双敲除小鼠与野生型进食饮水、体重增长比较。结果 结论 调控，仔鼠初生重及泌乳期体重。为利用基因敲除小鼠研究多巴胺D1、D3受体的功能及它们之间的相互作用奠定坚实的基础。 关键词【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2014.010.000 Effect of DRD1, DRD3 gene knockout and double gene knockout on body weight in mice ZHANG Bao, XING Bo, WEI Shu-guang, JIA Xiao-ni, ZHANG Hong-bo, LI Sheng-bin （, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China） Abstract] Objective To study the effect of dopamine receptors on neurological and physiological activities. Methods Dopamine D1 receptor gene (DRD1) knockout mice, dopamine D3 receptor (DRD3) gene knockout mice were introduced, and double gene knockout mice were bred in our lab. Seven SPF male mice in each group were used in this experiment. The food intake, water intake, body weight gain for 24 hours were tested on the age of 30 d, 50 d, and 70 d were determined, and were compared with those of wild type mice. Results DRD1 gene and DRD3 gene showed significant effect on the body weight in mice in age of 21 day and 35 day, but at the age of 90 day, the differences became insignificant among the mice of various genetypes. Conclusions Dopamine may effect on the foraging and satiety in newborn?mice through regulating the hypothalamic-pituitary-adrenal (HPA) axis activity, and finally leads to a reduced body weight gain in newborn mice and puppies during lactation. Furthermore, DRD1 gene and DRD3 gene may influence on body weight of newborn mice through regulating mothers’ lactation, lead to a lower body weight at ablactation, and compensatory increase of body weight after ablactation. Our results provide a substantial foundation for studying the function and interaction of DRD1 and DRD3 genes. [Key words] DRD1 gene, DRD3 gene