恶性乳腺肿瘤p53基因pcr-上海农林职业技术学院.docVIP

检测p53诊断的初步研究王彤光1,陈 谊1侯加法（1.上海农林职业技术学院上海松江201600；2. 渭南职业技术学院，陕西渭南：714000； 3. 南京农业大学动物医学院南京卫岗210095） 摘要：研究犬乳腺恶性肿瘤p53基因在体内的表达，探讨其与犬乳腺肿瘤恶性程度的相关性。根据Genbank中犬p53基因序列以及文献报道的高度保守区域，设计一对引物，同时以GAPDH基因序列作为内参，将临床采集的犬乳腺肿瘤组织进行p53基因PCR检测，分析p53基因的表达量变化趋势。结果表明，良性肿瘤组织p53基因的表达量极小；恶性肿瘤组织中的表达量随着其恶化程度明显增高。由此可推断，检测p53基因的表达量可作为诊断犬乳腺肿瘤、判断恶化肿瘤程度及预后的一个重要指标。关键词：犬；恶性乳腺肿瘤；P53基因；基因表达；PCR检测Preliminary Study on Diagnosis of Canine Mammary Tumor by Detection of p53 Gene Wang Tongguang1,3，Wang Weihua2，Chen Yi1，Hou Jiafa3 (1. Shanghai Vocational and Technical College of Agriculture and Forestry, Songjiang, Shanghai, 201600；2. Weinan College of Vocational and Technical, Weinan, Shaanxi, 714000；3. College of Veterinary Medicine, Nanjing Agricultural University, Weigang, Nanjing, 210095) Abstract: In order to explore the vivo expression of canine malignant mammary tumor related gene, study the relativity on gene expression and tumor malignancy. A pair of specific primer was designed according to the p53 gene sequence in Genbank and the hyperconservative region from reference reported, and acted the GAPDH gene sequence as the confidential reference. The p53 gene of mammary tumor was detected with PCR for analyzing the variation tendency of the expressive quantity. The results showed that the expressive quantity of p53 gene in benign tumor was minimal; the expressive quantity in malignant tumor could markedly increase with the impaired condition. So, it could be concluded that detection of p53 gene expressive quantity can act as an important indicator in diagnosis of canine mammary tumor，determination of its malignant tumor staging and the prognosis of. Key words: canine; malign mammary tumor; p53 gene; gene expression; PCR detection p53 基因表达异常是恶性肿瘤中最常见的基因损伤之一，其在犬乳腺肿瘤中表达量的多少，可作为犬恶性肿瘤诊断及预后的主要标志物之一[1]。p53基因是机体的抑癌基因，编码一种分子量为53 ku的蛋白质，p53基因的失活对肿瘤的形成起重要作用。但是p53也有其两面性，它除了能够抑制肿瘤发生外，还能够导致细胞的凋亡，正是由于机体精密的调控作用，细胞中的p53基因维持了一种比较适宜的表达量。在正常细胞中p53表达量较低，而在肿瘤细胞中其表达量大幅上调[2]。文献报道，p53基因的表达量随着肿瘤发展而逐步升高，因此p53 基因在犬乳腺中的表达量，可以作为一种