脂多糖诱导的copd模型大鼠肺支气管上皮mrp中国试验动物学报.docVIP

脂多糖诱导的COPD模型大鼠肺支气管上皮MRP1功能分析 汪珊珊1，汪电雷1,2*，陶秀华1，汪辰吟1，陈金佩1，杨丽丽1，曹 银 （1.安徽，安徽 合肥 230031；2.，安徽 合肥 230031） 收稿日期： 修回日期： 基金项目 国家重大新药创制资助项目(No 2009ZX09103-399)；国家自然科学基金资助项目(No；教育部科学技术重点项目 (No 210101)；安徽高校省级自然科学研究重点项目资助(No KJ2010A210，KJ2012A186） [作者简介] （198-），，硕士研究生，药物代谢动力学研究，Tel：，E-mail：@126.com [通讯作者] *汪电雷(1977- )，男，硕导，副教授，药物代谢动力学研究，Tel：(0551)5169230, E-maildlwang@ MRP1 expression and function of pulmonary bronchial epithelia in LPS-induced COPD rat model WANG Shan-shan1, WANG Dian-lei1,2*, TAO Xiu-hua1, WANG Chen-yin1, CHEN Jin-pei1, YANG Li-li1, CAO Yin3 ( 1, College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230031, China; 2, Laboratory of Drug Metabolism and Pharmacokinetics, Anhui University of Chinese Medicine, Hefei 230031, China; 3, Pharmacy Dept, Psychiatric hospital of Hefei 230022, China） Abstract Objective: Study the impact of lipopolysaccharide (LPS)-induced chronic obstructive pulmonary disease （COPD） rat model on the function of multidrug resistance-associated protein 1（MRP1） in pulmonary bronchial epithelia. Methods: Using LPS intratracheal instillation method to establish COPD rat model. Rats divided into 3 groups: (1) Normal control；(2) Modeling for 14 days after dosing LPS; (3) Modeling for 28 days after dosing LPS. Pulmonary function and the concentration of phenolsulfonphthalein in BALF and plasma were measured. The ratio of phenolsulfonphthalein concentration in BALF/plasma was used as index of the function of MRP1 in rat pulmonary bronchial epithelia and the expression of MRP1 in pulmonary bronchial epithelia was also observed with immunohistochemistry. Results: Compared with the normal group, the pulmonary functions of the rats in the progression of modeling were significantly reduced; after intravenous administration of phenolsulfonphthalein, the ratio of phenolsulfonphthalein concentration in BALF/plasma was gradually reduced; the expression of MRP1 in pulmonary bronchia