降钙素基因相关肽对脂多糖诱导的巨噬细胞表达髓样细胞触发受体-1的影响.pdfVIP

中南大学学报 学版) 2010 JC ，35(2) http：／／】b【y】．【xysm．net 100 entSouthUnivrMedSci) … ～ … ‘……。 · ARTICLES · ·论 著 · Effectofcalcitoningene-relatedpeptideontheexpression oftriggeringreceptorexpressedonmyeloidcells一1in lip0p0lysaccharide-inducedmacrophages LIYunchao，GUANChaxiang，ZHOUYang，SUNGuoying，SHIMeng，WUJing，LIWenjie (1．DepartmentofClinicalMedicine；2．D~artmemofPhysiology，XiangyaSchoolof Medicine，CentralSouthUniversity，Changsha410078，China) Abstract： 0bjective Todeterminetheeffectofcalcitoningene-relatedpeptide(CGRP)ontrig— geringreceptorexpressedonmyeloidcells一1(TREM-1)inthelipop0lysaccharide(LPS)一inducedmacro— phagesanditssignaltransductionpathway．Methods ThelevelsofTREM一』mRNA inthemacrophages wereobservedbyreversetranscription—polymerasechainreaction (RT—PCR)，andfloweytometrywas performedtodetectTREM一1proteinexpressionlevelsinthemacrophages．Results CGRPhadnoregu· latingeffectontheexpressionofTREM一1inthemacrophages：LPScouldup—regulatemacrophagestoex- pressTREM ·1；CGRP increasedTREM-1mRNA expressioninLPS—inducedmacrophagesindoseand time—dependentmanner；CGRP increasedTREM一1protein expression in LPS-induced macrophages， whichcouldbepartiallyreversedbyH-7orH一89 (P0．05)．Conclusion CGRPcanregulatethe LPS—inducedmacrophagessynthesisand secretion ofTREM一1，and theintracellularsignaltransduction pathwayisrelatedtoPKA andPKC． Keywords： calcitoningene—relatedpeptide； triggeringreceptorexpressedonmyeloidcells-1； lipopolysaceharide； macrophage 降钙素基因相关肽对脂多糖诱导的巨噬细胞 表达髓样细胞触发受体一1的影响 李云超 ，管茶香 ，周漾 ，孙 国瑛 ，施