小孢子.pdfVIP

Plant Cell Rep (2002) 21:105–111 DOI 10.1007/s00299-002-0482-8 CELL BIOLOGY AND MORPHOGENESIS A. Gémes-Juhász · P. Balogh · A. Ferenczy Z. Kristóf Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber (Cucumis sativus L.) Received: 26 November 2001 / Revised: 30 April 2002 / Accepted: 2 May 2002 / Published online: 19 July 2002 © Springer-Verlag 2002 Abstract Gynogenic plants of pickling cucumber were Abbreviations BA : 6-Benzylaminopurine · successfully produced by in vitro culture from unpolli- NAA : α-Naphthaleneacetic acid · TDZ: Thidiazuron nated ovules. Haploids and spontaneous doubled haplo- ids originated directly through embryogenesis. The cu- cumber ovaries were placed on induction media contain- Introduction ing thidiazuron and cultured in the dark for 2–5 days, at 24°C, 28°C or 35°C. After induction, the material was Cucumber is a monoecious, open-pollinated crop. In transferred to regeneration media containing α-naphtha- modern agriculture, F1 hybrids are almost exclusively leneacetic acid and 6-benzylaminopurine. Results from bred. One partner in the cross is always a wholly female the regeneration test indicated that the heat treatment in- (gynoecious) line, which can be maintained by treatment creased the efficiency of gynogenesis in the optimal de- with silver-thiosulphate, while the other partner is gener- velopmental stage of the female gametophyte. The high- ally a monoecious line (with mixed flowers) although it est number of embryos occurred following the35°C in- may also be a gynoecious line (e.g. in the case of par- duction treatment. The maximum frequency of gynogen- thenocarpic hybrids). The parenta