中药瓦松粗提物抗菌效应（Chinese medicine Wasong extracts antibacterial effect）.doc

中药瓦松粗提物抗菌效应（Chinese medicine Wasong extracts antibacterial effect） Chinese medicine Wasong extracts antibacterial effect Update Date: 2010-12-22 Click: Cai Yuying, Zhang Weiwei, Guang Guang, He Zuze Abstract: through the traditional Chinese medicine antibacterial test Wasong extracts on clinical isolates of gram positive bacteria and gram negative bacteria, confirmed that Wasong extract has obvious antibacterial effect. Keywords Wasong extracts; antibacterial The, Anti-bacteria, Effects, of, the, Rough, Extract, from, The, Chinese,, Traditional, Herb, Wasong Cai, Yuying, Zhang, Wei, Wei, Xingguang, et, Al Institute, of, Materia, Medica, Shandong, Provincial, Academy, of, Medical, Sciences, Jinan 250062 Abstract By the experimental research of the killing effects of the rough extracts of Chinese traditional drug Wasong (Orastachys Fimbriatus) - the extracts to Gand, was proved to have good anti-baceria effects. Key, words, Rough, extract, of, Orastachys, Fimbriatus, Anti-bacteria, effects Chinese medicine Wasong extracts by Gram positive bacteria (Staphylococcus aureus, Bacillus subtilis, Staphylococcus aureus) and gram negative bacteria (such as citric acid bacillus, Escherichia coli, Pseudomonas aeruginosa, proteus, paratyphoid bacillus, Bacillus dysenteriae and aerobacteraerogenes) a total of 10 species and 216 strains. By using plate method in vitro antibacterial effect test, and provide scientific basis for clinical treatment. 1 materials and methods 1.1 medium 1.1.1 beef extract medium: 0.5% beef extract, 0.5% sodium chloride, 1% peptone and 1% glucose broth, pH7.6 prepared by high pressure steam sterilization of 20 min (10 pounds), as the dilution broth; cultivating Shigella plus 1% calf serum. 1.1.2 meat agar medium: 2% agar was added to beef paste medium (broth), and autoclaved as a sensitive test for clinical isolates. 2 test bacteria and liquid preparation A clinical isolate of tested strains. 216 strains were Staphylococcus aureus, Staphylococcus aureus,