用适应性实验对ICR、BALBc和C57BL6.PDFVIP

动 物 学 研 究 2008，Feb. 29(1) ：49 －55 CN 53-1040/Q ISSN 0254-5853 Zoological Research 用适应性实验对 ICR、BALB/c 和 C57BL/6 小鼠探索行为及记忆能力的评价 施俊巍，邹 洪，金玫蕾* ( 中国科学院上海生命科学研究院，上海 200233) 摘要：用洞板仪和旷场行为红外线检测系统对远交群ICR 小鼠、近交系BALB/c 小鼠和近交系C57BL/6 小 鼠的探洞、直立和跨格行为进行了测试，并通过适应性实验比较了它们的探索行为和记忆能力。研究发现：在各 项试验中，ICR 小鼠表现出最强的探索能力，而C57BL/6 小鼠的探索能力最差；ICR 小鼠还表现出比另外两个品 系小鼠更强的对新环境的适应性，其记忆也明显优于C57BL/6 小鼠。实验结果提示：小鼠探洞行为的适应性实验 可以用来测试小鼠的记忆能力，而小鼠的直立行为在一定程度上也反映了其对周围环境、事物的探索和认知。这 些结果为今后在研究小鼠神经生物学时的行为学指标选择问题提供了参考依据，同时也提示了在进行小鼠行为学 实验时，根据不同研究目的选择不同品系的重要性。 关键词：动物行为学；品系；探索行为；适应性；记忆能力 中图分类号：Q958.12 ；Q189 文献标识码：A 文章编号：0254-5853-(2008)01-0049-07 Assessing Exploratory Behavior and Memory in ICR, BALB/c and C57BL/6 Mice Using Habituation * SHI Jun-wei, ZOU Hong, JIN Mei-lei (Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200233) Abstract: Differences in exploratory, rearing and locomotion activity of ICR, BALB/c and C57BL/6 strain were investigated by a 16-hole board task and an open field with an automated infrared detecting system. ICR mice had the highest response rate while C57BL/6 mice had the lowest. It took less time for ICR mice to get used to a new environment and its performance in memory testing was also superior to C57BL/6 mice. The results confirmed the feasibility of using a hole-board test to assess memory. Comparisons between the trend of rearing and hole-poking activity demonstrated that the rearing behavior of mice, to a certain deg