生物导论（The introduction of biological）.docVIP

生物导论（The introduction of biological） Introduction to biotechnology The first chapter is the introduction 1. meaning: Biotechnology Biotechnology (Biotechnology), also known as biological engineering (bioengingineering), refers to the people on the basis of modern life science, combined with the principle of science engineering advanced technology and other basic subjects, in accordance with the pre design or modification of biological processing of biological raw materials for the production of human or reach the required product a certain purpose. Advanced biological technology which refers to the new technology of gene engineering, cell engineering, enzyme engineering, fermentation engineering etc.. The second chapter of gene engineering 1. concepts of gene engineering; gene engineering and genetic engineering or genetic manipulation, refers to the willingness of people using artificial method in vitro reorganization of various biological DNA, a new combination of genetic material, and make it sustainable and stable propagation in receptor cells, resulting in a large number of new species of technology. The 2. elements of gene engineering; gene engineering is the largest at the molecular level, the expression on the cellular level. Four necessary conditions including the implementation of gene engineering; gene, enzyme, 1 carrier molecule, cell receptor. The basic procedure of gene engineering; (1) from the organism complex genome, isolated a gene fragment of DNA. (2) in vitro, with the purpose of the DNA is connected to the park is able to reproduce and select vector of molecular markers, the formation of recombinant DNA molecules. (3) the recombinant DNA molecule into the cell receptor. (4) with recombinant DNA cells to obtain a large number of cell populations. (5) from a large number of reproductive cell colonies, cell clones were screened with the recombinant DNA molecule. (6) the gene clones selected for further analysis (7) the cloning of gene expression ve