基于RTNs分子标记的虎杖地理属性研究.pdfVIP

ofAnhui 安徽农业科学，Journal A咖．Sci．2013，41(31)：12266—12268，12270 责任编辑石金友责任校对卢瑶 基于RTNs分子标记的虎杖地理属性研究 甘晓燕，陈静，陈新4 (成都中医药大学药学院，四川成都611137) 为阈值，可将供试材料分为5类。多样性分析结果表明，虎杖种质资源在分子水平上存在较大差异，基于iPBS标记的虎杖基因型与地 理属性有密切关系。[结论]试验研究了虎较的地理属性，可为虎杖种质资源分类和深入研究植物与环境的关系提供参考。 关键词虎杖；反转录转座子；iPBS；地理属性 中图分类号S188文献标识码A 文章编号0517—6611(2013)31—12266—03 MolecularMarkersforIdentificationof Distributionof Retrotransposon·based Geographical Po咖gonumcuspidatum 1 GAN etal of ofT】麓ditionalChinese 61 Xiao-yan(ScboolPharmacy，ChengduUniversity Medicine，Chengdu，Sichuan137) the distributionof 9 Abstract[Objective]Tostudygeographical Polygonumcupidatum．[Method]11legenotypiepolymorphismamong resources from6areasinSichuanProvincewasstudiedwithiPBS Reset22iPBS of germplasm Polygonumcuspidatum analysis．1 primers with werescreenedoutfrom50iPBS thetotal161identified higherpolymorphism primers：66．5％bandsdisplayedpolymorphismamong bands．Each an of4．86 cluster showsthatthematerialscanbe primeramplifiedaverage polymorphicfragment．Theanalysis separatedby iPBS GS to0．79．allaccessionscanbeascribedinto5 resultsshowthat onmolecular analysis．Whenequal groups．The highgeneticdiversity is is to level the resourcesof related cupidatumexisted；genotypeclosely geographical amonggermplasm Polygonum of was will referencefor resourcesclassificationof distribution sion]111egeographical Polygonumcupidatums