电针对阿尔茨海默病大鼠学习记忆力改善及海马神经元损伤保护的作用及机制.PDFVIP

·682· Shanghai J Acu-mox, Sep 2012, Vol 31, No 9 文章编号:1005-0957(2012)09-0682-04 ·动物实验· 电针对阿尔茨海默病大鼠学习记忆力改善及海马神经元损伤 保护的作用及机制 国海东,邵水金,朱晶,陆萍萍 (上海中医药大学基础医学院解剖教研室,上海 201203) 【摘要】 目的 探讨电针保护海马神经元和改善阿尔茨海默病(Alzheimer’s disease,AD)大鼠学习记忆力的作用机制。 方法 将大鼠随机分为正常组、假手术组、模型组和电针组,采用 Morris 水迷宫检测空间学习和记忆能力的变化。 Hoechest33342染色观察各组海马神经元的凋亡;Western blot检测Bcl-2 和Bax的水平;免疫组织化学染色分析Caspase-3 阳性神经元的个数和光密度值。结果 电针治疗可明显提高AD大鼠学习和记忆功能,并可降低海马CA1区凋亡细胞的数目、 上调 Bcl-2 的表达和下调 Bax 和凋亡执行蛋白 Caspase-3 的表达。结论 电针可通过调节 Bax 和 Bcl-2 的平衡比值,降低 Caspase-3的表达,对抗β-淀粉样蛋白诱导的神经元凋亡,改善AD大鼠学习和记忆功能。 【关键词】 针刺疗法;电针;阿尔茨海默病;大鼠 【中图分类号】 R2-03 【文献标志码】 A DOI:10.3969/j.issn.1005-0957.2012.09.682 Mechanism of Action of Electroacupuncture in Improving Learning and Memory and Protecting Injured Hippocampal Neuron in Alzheimer’s Disease Rats GUO Hai-dong, SHAO Shui-jin , ZHU Jing, LU Ping-ping . Teaching and Research Section of Anatomy, Shanghai University of Traditional Chinese School of Basic Medicine,Shanghai 201203,China [Abstract] Objective To investigate the mechanism of action of electroacupuncture in improving learning and memory and protecting injured hippocampal neuron in Alzheimers disease (AD) rats. Method The rats were randomized into normal, sham operation, model and electroacupuncture groups. Spatial learning and memory were assessed using a Morris water maze. Change of learning and memory abilities was detected by Morris water maze. Apoptosis of hippocampal neurons was examined by hoechest 33342 staining. Bcl-2 and Bax levels were measured by Western blot analysis. The number and optical density value of Caspase-3-positive neurons were determined by Immunohistochemical staining. Result Electroacupuncture can significantly improve AD rat learning and memory func