草甘膦对小鼠睾丸支持细胞的毒作用及机制研究 - 第三军医大学学报.docVIP

茶多酚对草甘膦致小鼠睾丸支持细胞氧化损伤及凋亡的保护作用 赵文红1，俞 慧1，张建国2，江城梅1 （233030安徽 蚌埠TUNEL法检测细胞凋亡情况；检测细胞中超氧化物歧化酶（super oxidase dimutase,SOD）活性、谷胱甘肽（glutathione ,GSH）及丙二醛（malondialdehyde ,MDA）含量。结果 GLY处理组细胞出现收缩变小、脱落、甚至破碎，细胞的存活率明显低于对照组（P0.05），为对照组的47.03%；细胞内LDH活性明显高于对照组；细胞凋亡率为（36.96±4.0）%，凋亡细胞数明显高于对照组（P0.05）；细胞内SOD活性、GSH含量与对照组相比明显降低，而MDA含量明显升高（P0.05）。各拮抗组与草甘膦组相比，细胞形态有不同程度改善，尤其是拮抗组4（TP80 mg/L）细胞生长状况明显好转，碎片明显减少；细胞的存活率、SOD活性及GSH含量明显升高（P0.05或P0.01）；细胞凋亡率、LDH活及MDA含量明显下降（P0.05或P0.01）。结论 GLY对小鼠sertoli细胞具有诱导细胞凋亡及抑制细胞增殖，降低细胞抗氧化能力的影响，TP对GLY这些损伤有一定的保护作用。 [关键词] 茶多酚；草甘膦；小鼠睾丸支持细胞；氧化损伤；凋亡 The protective study of tea polyphenol on oxidative damage and apoptosis of sertoli cell of mouse induced by glyphosate Zhao Wenhong1,Yu Hui1,Zhang Jianguo2 ,Jiang Chengmei1 (1Department of Preventive Medicine , 2the First Affiliated Hospital, Bengbu Medical College, Bengbu, AnHui Province, 233030, China) [Abstract] Objective To investigate the protective effect of different dose of tea polyphenol(TP) on sertoli cell of mouse against the oxidative damage and apoptosis induced by glyphosate(GLY). Methods The oxidative damage model of primary sertoly cell of mouse was established through GLY(90 mg/L) incubation for 24h. Four rivalry groups were formed through treatment of GLY(90 mg/L) and different dose of TP (10 mg/L、20 mg/L、40 mg/L and 80 mg/L).Meanwhile , the control group was incubated with normal medium .After incubation for 24h the sertoli cells of every group were observed under inverted microscope .The proliferation rate was evaluate by MTT assay . The cell cytotoxicity was measured by LDH test .The state of apoptosis of cells was explored with TUNEL. The activity of super oxidase dimutase(SOD),as well as the levels of glutathione (GSH）and malondialdehyde (MDA）were detected . Results when sertoli cells were treated with GLY (90 mg/L) the cells became smaller ,cast-off cells and cell breakage increased. The cell proliferation was inhibited by 47.03%. The activity of lactate dehydrogenase(LDH) and the level