如何通过径向流层析技术提高速度.pdfVIP

Superflo Radial Flow Chromatography Columns Sepragen Corporation Problems with Conventional Column Scale Up  Slow flow rates and large cycle times  Greater resin usage for same productivity  Bigger columns and Large Footprint  Performance compromise on scale up  Packing is unwieldy and requires investment in infrastructure  High Purification costs Sepragen Corporation 2 Key Benefits of Superflo Radial Flow Columns Proven, Superflo RFC Columns enable:  Shorter cycle times for Protein A, IEX, Affinity and HIC steps  Small Footprint  4 – 10 X higher flow rates per sq m.  Low operating pressures.  Easy, Rapid, Consistent slurry packing and unpacking.  Superior HETP, better recovery and lower pool volume  Rapid linear scale-up  Reduce purification costs Sepragen Corporation 3 How it Works Pressure relief valve Radial distribution on sample inlet prevents channels allow uniform column damage (not distribution of flow to outer shown in drawing). wall of column. Bubble trap prevents drying of Packing ports provide