儿童青少年脑容积比内表型的双生子研究.docVIP

儿童青少年脑容积比内表型的双生子研究 马兴顺1 ，傅一笑1 ，胡小梅1，蒙华庆1，李 涛2，邓 伟2，侯 枭4，张晓薇2，林 鄞2，雷 威2，李名立2，康林娥3，黄永峰3，王 娜3，邱 田1，李 晓1，刘 洋1 （400016重庆，重庆医科大学附属第一医院心理卫生中心1；610041成都，四川大学华西医院心理卫生中心2；719000，陕西 榆林，陕西省榆林市第一医院神经内二科3；400000，重庆，重庆医药高等专科学校4） [摘要] 目的：探索儿童青少年脑灰白质容积比、灰质全脑容积比、白质全脑容积比是否为稳定内表型，作为儿童脑发育的关键内表型指标。方法：采用横断面设计，以学籍登记为线索募集12~18岁的双生子，采集静脉血液标本提取DNA，进行卵型鉴定。用3.0T MRI（GE）对双生子对进行头颅结构扫描，采集全脑166层矢状位图像。结果：①最终符合条件58对双生子成功提取DNA，卵型鉴定结果显示：同卵双生子31对，异卵双生子27对；②Holzinger方法估计灰白质容积比遗传度0.828，灰质全脑容积比遗传度为0.596，白质全脑容积比遗传度为0.741。结论：灰白质容积比有较高遗传度，说明灰白质容积比有相对稳定的遗传基础，可以作为儿童青少年脑发育的内表型指标。 [关键词]双生子；儿童青少年；内表型；灰质容积；白质容积；容积比 To Explore the endophenotype of brain volume ratio in children and adolescents: a twin study Ma Xingshun1,Fu Yixiao,Hu Xiaomei1,Meng Huaqing1 ,Li Tao2,Deng Wei2,Hou Xiao4,Zhang Xiaowei2,Lin Yin2,Lei Wei2,Li Mingli2,Kang Line3,Huang Yongfeng3,Wang Na3,Qiu Tian1,Li Xiao1,Liu Yang1 (1Mental Health Center.The First Affiliated Hospital of Chongqing Medical University.Chongqing，400016；2Mental Health Center-West China Hospital of Sichuan University.Chengdu.sichuan.China 610041；3The Second Department of neurology.The First Hospital of Yulin,Yulin,Sanxi，719000；4Chongqing Medical and Pharmaceutical college.Chongqing.China 400000) [Abstract]Objective: To explore whether cerebral volume ratios such as gray-to-white matter, gray matter-to-global cerebral, white matter-to-global cerebral are stable endophenotype which can be used as key endophenotype index of children and adolescents brain development.Method: Using cross-sectional design,totally 58 pairs of twins aged 12~18 years were recruited according to the school registration information．DNA was extracted from venous blood samples to identify zygosity.With 3.0T MRI ( GE )we have obtained all 58 twin pairs brains structure MR imaging for each person 166 scans.Result: (1) 58 twin pairs DNA were picked up successfully extracted. zygote identified : monozygotic twin pairs:31, dizygotic twin pairs 27; (2)Holzingers method estimated heritabilities of three cerebral