基质金属蛋白酶与动脉粥样硬化性脑梗死初发及复发相关性研究.docVIP

基质金属蛋白酶与动脉粥样硬化性脑梗死初发及复发相关性研究 喻 明，李 凤，聂本刚，蒋世杰，方登富，陈小容，何晓非（629000四川 遂宁，遂宁市中心医院神经内科） [ [摘要] 目的：研究基质金属蛋白酶9（MMP-9）R279Q和R668Q多态性与动脉粥样硬化性脑梗死初发及复发的相关性。方法：选取初发脑梗死350例，复发脑梗死340例，正常健康组360例。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析法对MMP-9基因R279Q和R668Q多态性进行分析；ELISA法检测MMP-9的含量，比较三组间MMP-9基因多态性频率分布及含量的差异。结果：初发脑梗死组及复发脑梗死组血清MMP-9水平显著高于对照组[(1.63±0.53）vs (0.48±0.24）mg/L, P＜0.01；（1.98±0.94）vs（0.48±0.24）mg/L, P＜0.01],[（1.98±0.94）vs (1.63±0.53) mg/L， P＜0.01]MMP-9 R279Q中复发脑梗死组G等位基因频率明显升高，与初发脑梗死组相比（62.5% vs 55.6% , χ2=1.951，P＞0.01）（62.5% vs 49.2%,χ2=8.121，P＜0.01）（55.6% vs 49.2%,χ2=2.035，P＞0.01）MMP-9 R668Q基因型(χ2=1.825，P＞0.01)（χ2=1.624，P＞0.01）Logistic回归分析显示，MMP-9 R279Q多态性是脑梗死复发的独立危险因素（OR:1.625，95%CI:1.072~2.566，P=0.005）MMP-9基因R279Q的G等位基因是脑梗死遗传易感基因之一，可能是脑梗死复发的潜在危险因素。 [关键词] MMP-9；基因多态性；脑梗死；复发 Correlations among the Matrix Metalloproteinase-9 Polymorphism and the atherosclerotic cerebral infarction for the first time onset and recurrence Yu Ming, Li Feng, Nie Ben Gang, Jiang Shi Jie, Fang Deng Fu,Chen Xiao Rong, He Xiao Fei. （Department of Neurology , the SuiNing central hospital .SuiNing 629000, China） [Abstract]Objective:R279Q and R668Q) and the atherosclerotic cerebral infarction for the first time onset and recurrence. Methods: The study consisted of 350 patients with atherosclerotic cerebral infarction for the first time onset, 340 patients with a recurrence of atherosclerotic cerebral infarction, 360 healthy subjects as control group. The serum level of MMP-9 was measured by enzyme-linked immunosorbent assay, and the MMP-9 R279Q and R668Q polymorphism were detected by PCR-restriction fragment length polymorphism. The results between the groups were analyzed. Results: The serum MMP-9 level in cerebral infarction groups were significantly higher than that in the control group [(1.63±0.53) vs (0.48±0.24)mg/L, P＜0.01; (1.98±0.94) vs (0.48±0.24) mg/L, P＜0.01].The serum MMP-9 level in the cerebral infarction recurrence group were significantly higher than those in the group of cerebral infarction for