PCNA及CD44v6在皮肤鳞状细胞癌及基底细胞癌中表达探究.docVIP

PCNA及CD44v6在皮肤鳞状细胞癌及基底细胞癌中表达探究[摘要]目的:探讨增殖细胞核抗原PCNA、细胞粘附因子CD44v6在皮肤鳞状细胞癌(Squamous cell carcinoma,SCC)和基底细胞癌(Basal cell carcinoma,BCC)中表达的意义及相关性。方法:采用免疫组织化学法对82例石蜡包埋组织标本进行检测和统计分析。结果:①PCNA在皮肤SCC中阳性率(90.5%)显著高于皮肤BCC组(52%)和正常皮肤组(13.3%),CD44v6在正常皮肤中的阳性率(100%)显著高于皮肤SCC组(66.7%)和皮肤BCC组(20%);②PCNA的高表达率与皮肤SCC的分化程度呈负相关,CD44v6的高表达率与皮肤SCC的分化程度呈正相关;③PCNA和CD44v6在皮肤SCC和BCC中表达无明显相关。结论:CD44v6和PCNA与皮肤BCC和SCC的发生、分化密切相关,可作为研究和判断皮肤SCC和BCC生物学行为和预后独立的重要指标。 [关键词]鳞状细胞癌;基底细胞癌;PCNA;CD44v6;免疫组织化学 [中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455(2010)10-1487-03 Study of the expressions of CD44v6 and PCNA in cutaneous squamous cell carcinoma and cutaneous basal cell Carcinoma WANG Qi,WANG Run-he,HU Qiang,JIN Jun-hua (1.Department of Plastic Surgery, 2.Department of Dermatology, the Center Hospital of Xiangfan,Xiangfan,441021,Hubei,China) Abstract: ObjectiveTo investigate the effects and relationship of the expressions of PCNA and CD44v6 in cutaneous squamous cell carcinoma and Cutoneous Basal cell carcinoma. MethodsThe expressions of CD44v6 and PCNA were evaluatecl by immunohistochemical assay in 42 cutanneous sqamous cell carcinoma,25 Cutaneous Basal cell carcinoma and 15 normal skin.Results① The positive expression rate of PCNA In CSCC (90.5%) was significantly higher than in CBCC (52%) and normal skin (13.3%) and differentiation (P0.05).ConclusionThe over expression of PCNA and the down expression of CD44v6 in CSCC and CBCC played an important role in occurrence 、invasion and metastasis of tumor. The expressions of CD44v6 and PCNA in CSCC and CBCC were not correlated,They can be used as the independent biological morkers for the occurrence and development of CSCC and CBCC. Key words: basal cell carcinoma;squamons cell carcinoma; PCNA; CD44v6; Immunohistochemical CD44是一类分布广泛的跨膜糖蛋白,参与机体各种生理功能,其外显子按表达和拼接方式的不同分为CD44s和CD44v[1],其中CD44v6与肿瘤侵袭和转移关系最为密切。增殖细胞核抗原PCNA是一种36KD的酸性核蛋白,为DNA聚合酶δ的辅因子,对DNA复制的调节起重要作用,PCNA的合成和表达与细胞增殖状态密切相关[2-3]。皮肤SCC和BCC均是皮肤角质形成细胞来源的肿瘤。其中SCC是皮肤恶性肿瘤中最常见的一种,占40%以上。CD44v6或P