带骨膜软骨及不带骨膜软骨自体异位移植实验探究.docVIP

带骨膜软骨及不带骨膜软骨自体异位移植实验探究[摘要]目的：通过软骨及带软骨膜软骨在未成年兔中的自体异位移植后变化的研究，为临床选择合适的移植物提供依据。方法：4周家兔12只，将兔的七、八、九肋软骨带骨膜取出，分别分为等长两段。一段保留骨膜，一段去除骨膜，分别埋置于兔的背部两侧对称部位。分别于4、8、12、16周分别取出所埋置的不同组的软骨。将软骨分别观察大体形态、测量长度、作组织学切片行HE染色及电镜观察其显微结构的变化。结果：4周时带骨膜软骨与周围组织结合较去骨膜软骨更紧密，16周与周围组织结合均紧密，两者无明显差异；4周时，两组软骨的长度无显著性差异，8周以后，带骨膜组较不带骨膜组更长（P＜0.05）； 4～8周两者均增生活跃，带骨膜比不带骨膜的增生更明显；12周后软骨纤维化明显，增生不明显。结论：幼兔中，带骨膜的软骨较不带骨膜的更适于自体移植。 [关键词]自体移植；骨膜；软骨 [中图分类号]R622[文献标识码]A[文章编号]1008-6455（2008）06-0864-03 The experimental study on the autograft cartilages with periosteum and without periosteum ZHANG Zhi-jia1,ZHANG Qing-guo2,ZHANG Jiao1 (Department of Plastic Surgery,the Southeast University Medicine College, Nanjing 210009,Jiangsu,China. 2.Plastic Surgery Hospital,Chinese Academy of Medical Science,Beijing 100041,China) Abstract： ObjectiveTo study the changes of the cartilage with and without the periosteum heterotopic transplantation in baby rabbit, in order to choose a suitable graft for clinical transplantation.Methods12 baby rabbits at the age of four weeks, each of them the seventh, eighth and ninth costal cartilages with periosteum were removed, and divided into two segments at the even length, one segment with periosteum the othernon-periosteum. They were buried under the skin of the back at bilaterally symmetrical parts. The different group of cartilages were took out at 4,8,12,16 weeks at random. The morphology of cartilage were observed and their length were measured.Their HE staining histological sections, and microstructure under the electron microscopy were observed.ResultsThe cartilages with periosteum integrate tightly with surrounding tissue at forth week while the cartilages without periosteum integrate tightly with surrounding tissue at sixteenth week and there was no significant difference at that time. The cells of cartilage growth actively at forth and eighth week, and those with periosteum were more actively than that without periosteal.They growthed not so actively and had more cartilage