槟榔促大鼠结肠平滑肌收缩量效及机制探析.docVIP

槟榔促大鼠结肠平滑肌收缩量效及机制探析摘 要:目的:观察槟榔对离体大鼠结肠平滑肌收缩运动的影响,并初步探讨其机制。方法:制备大鼠结肠平滑肌肌条,以9 g/L NaCl溶液 (NS)、槟榔为对照组,分别记录单独使用槟榔和阻断剂(酚妥拉明、维拉帕米和阿托品)孵育后槟榔引起的收缩效应。结果:不同浓度槟榔对离体大鼠结肠平滑肌的促进作用明显,与NS对照组比较,槟榔0.1g/L、1g/L、10g/L浓度时引起的肌条收缩最大振幅和面积均有统计学意义(P0.05)。结论:槟榔对大鼠结肠平滑肌的收缩活动有兴奋作用,且呈正相剂量关系,引起的收缩效应可被M受体阻断剂阿托品和Ca2+阻断剂维拉帕米阻断,而未能被α-受体阻断剂酚妥拉明阻断。 关键词:中药;槟榔;结肠;平滑肌;促进 中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)01-0141-03 Promotion dose-effect Relationship of Areca Seed on Murine Colonic Smooth Muscle Strips in Vitro and Its Mechanism LU Tao, WEI Mu?xin (Department of Traditional Chinese Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu, China) Abstract:Objective:To determine the effect of Areca Seed (AS) on murine colonic smooth muscle strips in vitro and to investigate its possible mechanism.Methods:The colonic longitudinal smooth muscle strips of rats were prepared. Control group was treated with sodium chloride (9g/L) and AS. We observed the effects of AS on the spontaneous contraction in muscle strips. We also observed its promotion effects on muscle strips fully incubated with Verapamil, Phentolamine and Atropine, respectively.Results:AS of different concentrations showed dose-dependent promotion effects on the spontaneous contraction of colonic muscle strips in vitro at the concentration of 1-1000g/L. Compared with NS group, the final concentration of AS 0.1g/L,1g/L and 10g/L, the peak value and area data of smooth muscle strips have significant difference (P　　 2.2 3种阻断剂孵育后对槟榔促进离体大鼠结肠平滑肌收缩效应的影响 以AS (100g/L)引起的平滑肌的收缩指标为100%,并作为对照组。各组最大收缩振幅结果:FTLM组(101.32±5.90)%(P=0.764),WLPM组(74.03±9.49)%(P=0.0001),ATP组(12.77±9.98)%(P=0.0001)。各组面积结果:FTLM组(98.16±3.41)%(P=0.676),WLPM组(73.10±11.92)%(P=0.0001),ATP组(11.45±8.54)%(P=0.0001)。与AS组相比,ATP组、WLPM组对肌条的收缩有抑制作用,其收缩振幅和面积的比较均有统计学意义(P0.05),见图2。 3 讨 论 槟榔又叫大腹子或海南子,为棕榈科植物槟榔成熟干燥种子,有驱虫消积、下气行水、截疟之功,可治疗食积气滞,腹痛胀满便秘等病症。历代本草多有记载,《药性论》:“宣利五脏六腑壅滞,破坚满气,下水肿。治心痛,风血积聚”。《新修本草》曰:“治腹胀,生捣末服,利水谷道”。《本草名言》曰:“槟榔入胸